Thompson R E, Hewitt C R, Piper D J, Hansen W P, Rubin R H, Tolkoff-Rubin N E, Barrett M C, Nelles M J
Clin Chem. 1985 Nov;31(11):1833-7.
This competitive immunoassay, based on inhibition by antigen of the idiotype-anti-idiotype interaction, detects adenosine deaminase binding protein (ABP), and involves use of monoclonal anti-idiotype antibodies prepared to a monoclonal antibody specific for ABP. The conditions for this new type of competitive immunoassay are investigated. This competitive immunoassay is as sensitive and reproducible as an earlier described "sandwich"-type immunoassay for ABP (Clin Chem 31: 679-683, 1985). Evaluation of urine samples from normal subjects and from patients showed increased concentrations of ABP in patients with renal disease.
这种竞争性免疫测定法基于抗原对独特型 - 抗独特型相互作用的抑制作用，可检测腺苷脱氨酶结合蛋白（ABP），并涉及使用针对ABP特异性单克隆抗体制备的单克隆抗独特型抗体。研究了这种新型竞争性免疫测定法的条件。这种竞争性免疫测定法与先前描述的用于ABP的“夹心”型免疫测定法（《临床化学》31: 679 - 683, 1985）一样灵敏且可重复。对正常受试者和患者的尿液样本评估显示，肾病患者体内ABP浓度升高。
