Thompson R E, Piper D J, Galberg C, Chan T H, Tolkoff-Rubin N E, Rubin R H
Clin Chem. 1985 May;31(5):679-83.
This enzyme immunoassay detects adenosine deaminase binding protein (ABP), a glycoprotein that is shed from the brush border of the proximal tubule in kidney damage. Two monoclonal antibodies, URO-4 and URO-4a, each react with different epitopes on ABP and are used as the "sandwich" pair of antibodies. A linear standard curve can be generated by using partly purified ABP isolated from the urine of patients with kidney disease. Release of ABP into the urine appears to reflect the severity of the insult to the nephron. Therefore, measurement of ABP in urine may help distinguish between tubular disease and glomerular disease and indicate renal allograft rejection in renal-transplant patients.
这种酶免疫测定法可检测腺苷脱氨酶结合蛋白（ABP），这是一种糖蛋白，在肾脏损伤时从近端肾小管的刷状缘脱落。两种单克隆抗体，URO - 4和URO - 4a，分别与ABP上不同的表位发生反应，并用作“夹心”抗体对。使用从肾病患者尿液中分离出的部分纯化的ABP可生成线性标准曲线。ABP释放到尿液中似乎反映了肾单位受损伤的严重程度。因此，检测尿液中的ABP可能有助于区分肾小管疾病和肾小球疾病，并提示肾移植患者的肾移植排斥反应。
