Villacampa Pilar, Menger Katja E, Abelleira Laura, Ribeiro Joana, Duran Yanai, Smith Alexander J, Ali Robin R, Luhmann Ulrich F, Bainbridge James W B
Division of Genetics, UCL Institute of Ophthalmology, University College London, London, United Kingdom.
PLoS One. 2017 Jun 26;12(6):e0179759. doi: 10.1371/journal.pone.0179759. eCollection 2017.
Retinal ischemia and pathological angiogenesis cause severe impairment of sight. Oxygen-induced retinopathy (OIR) in young mice is widely used as a model to investigate the underlying pathological mechanisms and develop therapeutic interventions. We compared directly the conventional OIR model (exposure to 75% O2 from postnatal day (P) 7 to P12) with an alternative, accelerated version (85% O2 from P8 to P11). We found that accelerated OIR induces similar pre-retinal neovascularization but greater retinal vascular regression that recovers more rapidly. The extent of retinal gliosis is similar but neuroretinal function, as measured by electroretinography, is better maintained in the accelerated model. We found no systemic or maternal morbidity in either model. Accelerated OIR offers a safe, reliable and more rapid alternative model in which pre-retinal neovascularization is similar but retinal vascular regression is greater.
视网膜缺血和病理性血管生成会导致严重的视力损害。幼鼠氧诱导性视网膜病变（OIR）被广泛用作研究潜在病理机制和开发治疗干预措施的模型。我们直接比较了传统的OIR模型（从出生后第7天（P）到第12天暴露于75%氧气）和另一种加速版本（从P8到P11暴露于85%氧气）。我们发现加速OIR诱导相似的视网膜前新生血管形成，但视网膜血管消退更严重且恢复更快。视网膜胶质增生的程度相似，但通过视网膜电图测量的神经视网膜功能在加速模型中得到更好的维持。我们在两种模型中均未发现全身或母体发病情况。加速OIR提供了一种安全、可靠且更快速的替代模型，其中视网膜前新生血管形成相似，但视网膜血管消退更严重。
