甘露糖-6-磷酸受体组装到重组网格蛋白衣被中。
Assembly of the mannose-6-phosphate receptor into reconstituted clathrin coats.
作者信息
Pearse B M
出版信息
EMBO J. 1985 Oct;4(10):2457-60. doi: 10.1002/j.1460-2075.1985.tb03956.x.
Abstract
In ionic conditions in which clathrin coats are stable, the mannose-6-phosphate receptor associates with the 100-kd/50-kd coat complexes purified from bullock brain coated vesicles. These aggregates exist as striking spherical structures of 300-1000 A diameter. When clathrin is included in the assembly mixture, cages are formed which apparently encapsulate these aggregates, giving, in the absence of lipid, structures resembling full coated vesicles.
摘要
在网格蛋白包被稳定的离子条件下，甘露糖-6-磷酸受体与从公牛脑包被小泡中纯化的100kd/50kd包被复合物结合。这些聚集体以直径为300 - 1000埃的显著球形结构存在。当网格蛋白包含在组装混合物中时，会形成笼状结构，这些笼状结构显然包裹着这些聚集体，在没有脂质的情况下，形成类似于完整包被小泡的结构。
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