网格蛋白立方体：正常笼状结构的一种极端变体。
Clathrin cubes: an extreme variant of the normal cage.
作者信息
Sorger P K, Crowther R A, Finch J T, Pearse B M
出版信息
J Cell Biol. 1986 Oct;103(4):1213-9. doi: 10.1083/jcb.103.4.1213.
Clathrin triskelions form polyhedral cages with hexagonal and pentagonal faces when dialyzed against suitable assembly buffers. However, when the buffer is made 12% saturated in ammonium sulfate and the dialysis is performed at 4 degrees C, clathrin polymerizes into cubes. The cube is constructed from eight triskelions with one at each corner. The edge length of the cube is approximately 45 nm, equivalent to the length of the leg of a triskelion. Thus, each edge of the cube is composed of two antiparallel legs overlapping over their whole length. The interactions between the legs in the cube are a subset of those postulated to occur in cages. Indeed, the cube can be derived from a pentagonal dodecahedron by removing 12 of the 20 triskelions with only slight adjustment of the legs of the remaining triskelions. The cube forms regular arrays and appears to be a favorable species for crystallization of clathrin.
当用合适的组装缓冲液进行透析时，网格蛋白三脚复合体形成具有六边形和五边形面的多面体笼。然而，当缓冲液中硫酸铵饱和度达到12%并在4℃下进行透析时，网格蛋白会聚合成立方体。该立方体由八个三脚复合体构成，每个角上一个。立方体的边长约为45纳米，等同于一个三脚复合体腿部的长度。因此，立方体的每条边由两条反平行的腿在其整个长度上重叠组成。立方体中腿部之间的相互作用是假定在笼状结构中发生的相互作用的一个子集。实际上，通过移除20个三脚复合体中的12个，仅对其余三脚复合体的腿部进行轻微调整，就可以从五角十二面体得到立方体。立方体形成规则阵列，似乎是网格蛋白结晶的有利形式。
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