Jessus C, Huchon D, Friederich E, Francon J, Ozon R
Cell Differ. 1984 Oct;14(4):295-301. doi: 10.1016/0045-6039(84)90018-6.
The binding of microtubule associated proteins (MAPs) to free 80 S ribosomes isolated from Xenopus laevis oocytes inhibits in vitro tubulin assembly (Jessus et al., 1984). The inhibition of tubulin polymerisation was shown to be dependent upon GTP. The dose of GTP needed to induce 50% of the maximal effect was 0.5 mM. Furthermore, the inhibition is enhanced by pretreatment of the ribosomes with ATP-gamma-S, and partially abolished after phosphatase treatment, which strongly suggests that protein phosphorylation regulated the inhibitory effect. When fluorescent purified MAPs are microinjected into Xenopus laevis oocyte, they cap 1 h later the basal nuclear envelope; in contrast, when the fluorescent MAPs-ribosome complex is injected, the fluorescent MAPs remain in the cytoplasm and never reach the region underlying the nuclear envelope.
微管相关蛋白（MAPs）与从非洲爪蟾卵母细胞中分离出的游离80S核糖体的结合会抑制体外微管蛋白组装（杰苏斯等人，1984年）。已证明微管蛋白聚合的抑制作用依赖于GTP。诱导最大效应的50%所需的GTP剂量为0.5 mM。此外，用ATP-γ-S预处理核糖体可增强抑制作用，而磷酸酶处理后抑制作用部分消除，这强烈表明蛋白质磷酸化调节了抑制作用。当将荧光纯化的MAPs显微注射到非洲爪蟾卵母细胞中时，1小时后它们会覆盖核膜基部；相反，当注射荧光MAPs-核糖体复合物时，荧光MAPs会留在细胞质中，永远不会到达核膜下方的区域。
