异鼠李素通过激活 Nrf2 信号通路对 LPS 刺激的人牙龈成纤维细胞的抗炎作用。

Anti-inflammatory effects of isorhamnetin on LPS-stimulated human gingival fibroblasts by activating Nrf2 signaling pathway.

机构信息

Department of Stomatology, The Forth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, 150001, China.

Department of Stomatology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150001, China.

出版信息

Microb Pathog. 2018 Jul;120:37-41. doi: 10.1016/j.micpath.2018.04.049. Epub 2018 Apr 26.

DOI:10.1016/j.micpath.2018.04.049

PMID:29704670

Abstract

Periodontitis is a highly prevalent infective and inflammatory disease with an adverse impact on systemic health. Isorhamnetin, a flavonoid mainly isolated from Hippophae fhamnoides L. fruit, has been reported to have anti-inflammatory effect. This study aimed to investigate the anti-inflammatory effects and mechanism of isorhamnetin on lipopolysaccharide (LPS)-induced inflammatory response in human gingival fibroblasts (HGFs). The production of inflammatory mediators and the expression of proteins were measured by ELISA and western blot analysis. The results demonstrated that isorhamnetin attenuated LPS-induced release of PGE, NO, IL-6, and IL-8 in HGFs. Isorhamnetin also inhibited LPS-induced NF-κB activation. The expression of Nrf2 and HO-1 were up-regulated by treatment of isorhamnetin. Furthermore, knockdown of Nrf2 by siRNA reversed the anti-inflammatory effects of isorhamnetin. In conclusion, these results suggested that isorhamnetin inhibited LPS-induced inflammation in HGFs by activating Nrf2 signaling pathway.

摘要

牙周炎是一种高发的感染性和炎症性疾病，对全身健康有不良影响。山奈酚是一种主要从沙棘果实中分离出来的类黄酮，已被报道具有抗炎作用。本研究旨在探讨山奈酚对脂多糖（LPS）诱导的人牙龈成纤维细胞（HGFs）炎症反应的抗炎作用及其机制。通过 ELISA 和 Western blot 分析测量炎症介质和蛋白的表达。结果表明，山奈酚可减轻 LPS 诱导的 HGFs 中 PGE、NO、IL-6 和 IL-8 的释放。山奈酚还抑制 LPS 诱导的 NF-κB 激活。山奈酚处理可上调 Nrf2 和 HO-1 的表达。此外，用 siRNA 敲低 Nrf2 可逆转山奈酚的抗炎作用。总之，这些结果表明，山奈酚通过激活 Nrf2 信号通路抑制 LPS 诱导的 HGFs 炎症。