在苏丹东部疟疾传播不稳定地区，匹配的外周血、胎盘血和脐带血中的基因型。

genotypes in matched peripheral, placental and umbilical cord blood in an area characterised by unstable malaria transmission in eastern Sudan.

作者信息

Fadlelseed Omar E, Osman Maha E, Shamseldin Nahla M, Elhussein Amar B, Adam Ishag

机构信息

Medical College, Bahri University, Bahri, Sudan.

Commission for Biotechnology and Genetic Engineering National Centre for Research, Khartoum, Sudan.

出版信息

Heliyon. 2017 Jun 15;3(6):e00326. doi: 10.1016/j.heliyon.2017.e00326. eCollection 2017 Jun.

DOI:10.1016/j.heliyon.2017.e00326

PMID:28653041

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5476470/

Abstract

BACKGROUND

There are few published studies on genotypes in peripheral, placental and umbilical cord blood in areas characterised by unstable malaria transmission.

METHOD

A cross-sectional study was conducted to investigate genotypes in matched peripheral, placental and umbilical cord blood in eastern Sudan. Thick blood smears and merozoite surface protein 1 (MSP1) and 2 (MSP2) genes as polymorphic markers in polymerase chain reactions were investigated in 3 kinds of samples of 153 pregnant women at delivery.

RESULTS

There was no significant difference in the prevalence of blood film-detected in which 5 (3.3%), 7 (4.6%) and 3 (2.0%) (P = 0.437) of the 153 samples were determined to be -positive by microscopy for maternal peripheral, placental and cord blood samples, respectively. Out of these 145 samples, 24 (16.6%), 39 (26.9%) and 24 (16.6%) (P = 0.039) of the peripheral, placental and cord samples, respectively, had submicroscopic parasitaemia (blood films were negative). There was no association between submicroscopic parasitaemia and age or parity. RO33 and K1 (MSP1 alleles) were detected in 21/29 (72.4%), 42/46 (85.7%), 26/27 (92.2%) and 6/29 (20.6), 16/46 (32.6) and 0(0) (P < 0.001) of the maternal, placental and cord samples, respectively. MAD20 was not detected in any of the samples. While the 3D7/IC1 allele was detected in 12 (41.3%), 30 (65.2%) and 4 (14.8%) (P < 0.001) of the peripheral, placental and cord samples, respectively, the FC (MSP2) allele was detected in only the 6 (20.6) placental samples. Multi-clonal infection was detected in 10 (34.4), 27 (58.6) and 3 (11.1) (P < 0.001) of the maternal placental and cord samples, respectively.

CONCLUSION

Compared with the peripheral and cord samples, placental samples had a higher prevalence of submicroscopic parasitaemia. MSP1 alleles were predominant in the cord, while MSP2 alleles were predominant in the placental samples, which had a significant higher multiplicity of the infection.

摘要

背景

在疟疾传播不稳定地区，关于外周血、胎盘血和脐带血中基因型的已发表研究较少。

方法

进行了一项横断面研究，以调查苏丹东部匹配的外周血、胎盘血和脐带血中的基因型。在153名分娩孕妇的3种样本中，通过厚血涂片以及聚合酶链反应中作为多态性标记的裂殖子表面蛋白1（MSP1）和2（MSP2）基因进行研究。

结果

血涂片检测到的患病率无显著差异，153份样本中分别有5份（3.3%）、7份（4.6%）和3份（2.0%）（P = 0.437）的产妇外周血、胎盘血和脐带血样本经显微镜检查确定为阳性。在这145份样本中，外周血、胎盘血和脐带血样本分别有24份（16.6%）、39份（26.9%）和24份（16.6%）（P = 0.039）存在亚显微镜下寄生虫血症（血涂片为阴性）。亚显微镜下寄生虫血症与年龄或产次之间无关联。RO33和K1（MSP1等位基因）分别在21/29（72.4%）、42/46（85.7%）、26/27（92.2%）的产妇样本、胎盘样本和脐带血样本中检测到，以及6/29（20.6%）、16/46（32.6%）和0（0%）（P < 0.001）。在任何样本中均未检测到MAD20。虽然3D7/IC1等位基因分别在12份（41.3%）、30份（65.2%）和4份（14.8%）（P < 0.001）的外周血、胎盘血和脐带血样本中检测到，但FC（MSP2）等位基因仅在6份（20.6%）胎盘样本中检测到。分别在10份（34.4%）、27份（58.6%）和3份（11.1%）（P < 0.0C）的产妇、胎盘和脐带血样本中检测到多克隆感染。