Tavangar Banafsheh, Arasteh Shaghayegh, Edalatkhah Haleh, Salimi Ali, Doostmohammadi Ali, Seyedjafari Ehsan
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Reproductive Biotechnology Research Center, Avicenna Research Institute, Tehran, Iran.
Artif Organs. 2018 Nov;42(11):E335-E348. doi: 10.1111/aor.12891. Epub 2017 Jun 27.
In this study, a ceramic-coated nanofibrous scaffold has been fabricated to biomimic the microstructure of natural extracellular matrix and the stiffening inorganic compartment of bone. Poly-l-lactic acid (PLLA) nanofibers were electrospun and exposed to oxygen plasma to induce hydrophilicity and promote ceramic adsorption. Hardystonite (HS), which possesses superior osteoinduction potential over hydroxyapatite, was coated on plasma-treated PLLA nanofibers by drenching the nanofibers in HS suspension. Pure and composite PLLA-based scaffolds were characterized in terms of physical and biological properties. In vitro cultivation of adipose-derived mesenchymal stem cells (AMSCs) on the scaffolds displayed that the composite scaffold is able to further support cell attachment and proliferation. In case of osteogenic differentiation of AMSCs, HS coating significantly increased the synthesis and activity of alkaline phosphate over 21 days period. In addition, the composite scaffold showed improved mineralization. The expression level of osteonectin and osteocalcin genes was significantly enhanced by HS coating of nanofibers. The biological improvement of PLLA nanofibrous matrix in the presence of HS nanoparticles could either be attributed to the release and stimulatory effect of constituent ions of HS or to the modification of chemico-physical properties of the resultant ceramic by silicon and zinc present in HS.
在本研究中，制备了一种陶瓷涂层纳米纤维支架，以模拟天然细胞外基质的微观结构和骨的硬化无机成分。聚-L-乳酸（PLLA）纳米纤维通过静电纺丝制备，并暴露于氧等离子体中以诱导亲水性并促进陶瓷吸附。与羟基磷灰石相比具有更高骨诱导潜力的硬硅钙石（HS），通过将纳米纤维浸泡在HS悬浮液中而涂覆在经等离子体处理的PLLA纳米纤维上。对纯的和复合的基于PLLA的支架进行了物理和生物学特性表征。在支架上对脂肪来源的间充质干细胞（AMSC）进行体外培养显示，复合支架能够进一步支持细胞附着和增殖。在AMSC进行成骨分化的情况下，HS涂层在21天内显著增加了碱性磷酸酶的合成和活性。此外，复合支架显示出矿化改善。纳米纤维的HS涂层显著提高了骨连接蛋白和骨钙素基因的表达水平。在存在HS纳米颗粒的情况下，PLLA纳米纤维基质的生物学改善可能归因于HS组成离子的释放和刺激作用，或者归因于HS中存在的硅和锌对所得陶瓷化学物理性质的改性。
