Department of Pharmaceutical Biomaterials, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
School of Chemistry, University College of Science, University of Tehran, Tehran, Iran.
J Biomed Mater Res A. 2019 Aug;107(8):1841-1848. doi: 10.1002/jbm.a.36707. Epub 2019 May 24.
The presence of inorganic bioactive minerals with polymers can accelerate and promote several processes including: bone cell joining, proliferation, differentiation, and expression of osteogenic proteins. In this study, zinc (Zn), copper (Cu), and imidazole metal-organic framework (MOF) nanoparticles were synthesized and coated over poly-l-lactic acid (PLLA) nanofibrous scaffolds for bone tissue engineering application. The surface and bioactive features of the scaffolds were characterized. The osteogenic potential of the scaffolds on human adipose tissue-derived mesenchymal stem cells (MSCs) was evaluated. Zn-Cu imidazole MOF coated PLLA scaffolds (PLLA@MOF) showed a comparable rate of MSC proliferation with the pure PLLA scaffolds and tissue culture plate (TCP). However, the PLLA@MOF potential of osteogenic differentiation was significantly greater than either pristine PLLA scaffolds or TCP. Hence, coating Zn-Cu imidazole MOF has a significant effect on the osteogenesis of MSC. Therefore, PLLA@MOF is novel scaffolds with bioactive components which are crucial for osteoconductivity and also able to provoke the osteogenesis and angiogenesis.
骨细胞的连接、增殖、分化和骨生成蛋白的表达。在这项研究中，锌（Zn）、铜（Cu）和咪唑金属有机骨架（MOF）纳米粒子被合成并涂覆在聚左旋乳酸（PLLA）纳米纤维支架上，用于骨组织工程应用。对支架的表面和生物活性特征进行了表征。评估了支架对人脂肪组织来源间充质干细胞（MSCs）的成骨潜力。Zn-Cu 咪唑 MOF 涂覆 PLLA 支架（PLLA@MOF）在 MSC 增殖方面与纯 PLLA 支架和组织培养板（TCP）相当。然而，PLLA@MOF 促进成骨分化的潜力明显大于纯 PLLA 支架或 TCP。因此，Zn-Cu 咪唑 MOF 的涂层对 MSC 的成骨有显著影响。因此，PLLA@MOF 是具有生物活性成分的新型支架，这些成分对于骨传导性至关重要，并且能够引发成骨和血管生成。
