一种氧化锌修饰的羟基磷灰石基水泥促进了牙本质上新的结晶化学计量和非晶态磷灰石的沉淀。
A zinc oxide-modified hydroxyapatite-based cement facilitated new crystalline-stoichiometric and amorphous apatite precipitation on dentine.
机构信息
Department of Stomatology, Dental Materials Section, Faculty of Dentistry, University of Granada, Granada, Spain.
Biomaterials Department, University of La Havana, La Havana, Cuba.
出版信息
Int Endod J. 2017 Dec;50 Suppl 2:e109-e119. doi: 10.1111/iej.12807. Epub 2017 Jul 31.
AIM
To evaluate the remineralization ability of two endodontic sealer cements.
METHODOLOGY
Mid-coronal dentine surfaces were subjected to: (i) 37% phosphoric acid (PA) or (ii) 0.5 mol L ethylenediaminetetraacetic acid (EDTA) conditioning prior to the application of two experimental hydroxyapatite-based cements, containing sodium hydroxide (calcypatite) or zinc oxide oxiapatite respectively. Samples were stored in simulated body fluid for 24 h or 21 days. Remineralization of the dentine surfaces were studied by Raman spectroscopy (mapping with K-means cluster and hierarchical cluster analysis) was undertaken. Nanoroughness and collagen fibril width measurements were performed with an atomic force microscopy. ANOVA and Student-Newman-Keuls test were performed (α=0.05).
RESULTS
Phosphoric acid+oxiapatite promoted both the highest dentine mineralization (P < 0.05) and crystallographic maturity at the dentine surface. Noncrystalline amorphous-like apatites were also formed. Dentine treated with PA+calcypatite attained the roughest surface (P < 0.05) with minimal fibril width (P < 0.05). Cross-linking of collagen only became greater in the group PA+oxiapatite after 21 days. The maximum relative mineral concentration and structure of collagen linked to the amide I and ratio amide III/AGEs was obtained after using PA+calcypatite at 21-days time-point (P < 0.05). EDTA produced a lower stoichiometric hydroxyapatite (P < 0.05) with decreased maturity, at the expense of carbonate band widening, although it favoured the nucleation of carbonated calcium phosphate.
CONCLUSIONS
Dentine surfaces treated with PA+oxiapatite attained the highest dentine remineralization with both crystalline-stoichiometric and amorphous apatites, at 21 days. EDTA conditioning facilitated amorphous-bulk mineral precipitation. The amorphization was more intense after using oxiapatite and provided an ion-rich environment favouring in situ dentine remineralization.
目的
评估两种根管封闭剂的再矿化能力。
方法
将中段牙本质表面用 37%磷酸（PA）或 0.5mol/L 乙二胺四乙酸（EDTA）预处理，然后分别应用两种含氢氧化钠（钙磷灰石）或氧化锌氧磷灰石的实验性羟基磷灰石基水泥。将样本在模拟体液中储存 24 小时或 21 天。通过拉曼光谱（用 K-均值聚类和层次聚类分析进行映射）研究牙本质表面的再矿化情况。用原子力显微镜进行纳米粗糙度和胶原纤维宽度测量。采用方差分析和 Student-Newman-Keuls 检验（α=0.05）。
结果
磷酸+氧磷灰石促进了牙本质表面的最高矿化（P<0.05）和结晶成熟度。还形成了非晶态类似磷灰石。经 PA+钙磷灰石处理的牙本质表面最粗糙（P<0.05），胶原纤维宽度最小（P<0.05）。只有在 21 天后，PA+氧磷灰石组的胶原交联才会增加。用 PA+钙磷灰石处理 21 天后，获得了最大的相对矿化浓度和与酰胺 I 相连的胶原结构以及酰胺 III/AGEs 比值（P<0.05）。EDTA 产生了较低的化学计量羟基磷灰石（P<0.05），结晶成熟度降低，代价是碳酸盐带变宽，尽管它有利于碳酸磷灰石的成核。
结论
在 21 天时，用 PA+氧磷灰石处理的牙本质表面获得了最高的牙本质再矿化，既有结晶态的化学计量比磷灰石，也有无定形磷灰石。EDTA 处理有利于无定形大块矿化沉淀。在用氧磷灰石处理后，非晶化更为剧烈，提供了一个富含离子的环境，有利于原位牙本质再矿化。
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