Toledano Manuel, Aguilera Fátima S, Osorio Estrella, Cabello Inmaculada, Toledano-Osorio Manuel, Osorio Raquel
Faculty of Dentistry,Dental Materials Section,University of Granada,Colegio Máximo de Cartuja s/n,18071 Granada,Spain.
Microsc Microanal. 2015 Feb;21(1):214-30. doi: 10.1017/S1431927614013658. Epub 2014 Dec 11.
The purpose of this study was to evaluate if mechanical loading influences bioactivity and bond strength at the resin-dentin interface after bonding with Zn-doped etch-and-rinse adhesives. Dentin surfaces were subjected to demineralization by 37% phosphoric acid (PA) or 0.5 M ethylenediaminetetraacetic acid (EDTA). Single bond (SB) adhesive—3M ESPE—SB+ZnO particles 20 wt% and SB+ZnCl2 2 wt% were applied on treated dentin to create the groups PA+SB, SB+ZnO, SB+ZnCl2, EDTA+SB, EDTA+ZnO, and EDTA+ZnCl2. Bonded interfaces were stored in simulated body fluid for 24 h and tested or submitted to mechanical loading. Microtensile bond strength (MTBS) was assessed. Debonded dentin surfaces were studied by high-resolution scanning electron microscopy. Remineralization of the bonded interfaces was assessed by atomic force microscope imaging/nanoindentation, Raman spectroscopy/cluster analysis, and Masson's trichrome staining. Load cycling (LC) produced reduction in MTBS in all PA+SB, and no change was encountered in EDTA+SB specimens, regardless of zinc doping. LC increased the mineralization and crystallographic maturity at the interface; a higher effect was noticed when using ZnO. Trichrome staining reflected a narrow demineralized dentin matrix after loading of dentin surfaces that were treated with SB-doped adhesives. This correlates with an increase in mineral platforms or plate-like multilayered crystals in PA or EDTA-treated dentin surfaces, respectively.
本研究的目的是评估在使用掺锌的酸蚀冲洗粘结剂粘结后，机械加载是否会影响树脂-牙本质界面的生物活性和粘结强度。牙本质表面用37%磷酸（PA）或0.5M乙二胺四乙酸（EDTA）进行脱矿处理。将单键（SB）粘结剂——3M ESPE——含20 wt% ZnO颗粒的SB+ZnO和含2 wt% ZnCl₂的SB+ZnCl₂应用于处理过的牙本质上，以形成PA+SB、SB+ZnO、SB+ZnCl₂、EDTA+SB、EDTA+ZnO和EDTA+ZnCl₂组。粘结界面在模拟体液中储存24小时后进行测试或施加机械加载。评估微拉伸粘结强度（MTBS）。通过高分辨率扫描电子显微镜研究脱粘后的牙本质表面。通过原子力显微镜成像/纳米压痕、拉曼光谱/聚类分析和马松三色染色评估粘结界面的再矿化情况。加载循环（LC）使所有PA+SB组的MTBS降低，而EDTA+SB组的标本无论是否掺锌均未出现变化。LC增加了界面处的矿化和晶体成熟度；使用ZnO时效果更明显。三色染色显示，在用掺SB粘结剂处理的牙本质表面加载后，脱矿牙本质基质变窄。这分别与PA或EDTA处理的牙本质表面矿质平台或板状多层晶体的增加相关。
