Technical University of Denmark, Department of Photonics Engineering, Roskilde, Denmark.
University of Copenhagen, Cariology and Endodontics, Department of Odontology, Faculty of Health and Medical Science, Copenhagen N, Denmark.
J Biomed Opt. 2017 Jun 1;22(6):65004. doi: 10.1117/1.JBO.22.6.065004.
The objective of this study was to test the inactivation efficiency of two different light-based treatments, namely ultraviolet B (UVB) and ultraviolet C (UVC) irradiation, on Pseudomonas aeruginosa biofilms at different growth stages (24, 48, and 72 h grown). In our experiments, a type of AlGaN light-emitting diodes (LEDs) was used to deliver UV irradiation on the biofilms. The effectiveness of the UVB at 296 nm and UVC at 266 nm irradiations was quantified by counting colony-forming units. The survival of less mature biofilms (24 h grown) was studied as a function of UV-radiant exposure. All treatments were performed on three different biological replicates to test reproducibility. It was shown that UVB irradiation was significantly more effective than UVC irradiation in inactivating P. aeruginosa biofilms. UVC irradiation induced insignificant inactivation on mature biofilms. The fact that the UVB at 296 nm exists in daylight and has such disinfection ability on biofilms provides perspectives for the treatment of infectious diseases.
本研究旨在测试两种不同的基于光的处理方法（即紫外线 B（UVB）和紫外线 C（UVC）照射）对不同生长阶段（24、48 和 72 小时生长）的铜绿假单胞菌生物膜的失活动力。在我们的实验中，使用了一种氮化铝镓发光二极管（LED）将紫外线照射到生物膜上。通过计算集落形成单位来量化 296nm 的 UVB 和 266nm 的 UVC 照射的有效性。作为对 UV 辐射暴露的函数，研究了不太成熟的生物膜（24 小时生长）的存活情况。所有处理均在三个不同的生物学重复中进行，以测试重现性。结果表明，UVB 照射比 UVC 照射更有效地灭活铜绿假单胞菌生物膜。UVC 照射对成熟生物膜的失活作用不明显。296nm 的 UVB 存在于日光中并且对生物膜具有这种消毒能力的事实为治疗传染病提供了新视角。
