Fan Fuqiang, Zhang Baiyu, Morrill Penny L
Northern Region Persistent Organic Pollution Control (NRPOP) Laboratory, Faculty of Engineering and Applied Science, Memorial University of Newfoundland, St. John's, NL A1B 3X5, Canada.
Earth Sciences, Faculty of Science, Memorial University of Newfoundland, St. John's, NL A1B 3X5, Canada.
Mar Pollut Bull. 2017 Sep 15;122(1-2):194-206. doi: 10.1016/j.marpolbul.2017.06.044. Epub 2017 Jun 24.
A method based on phospholipid fatty acid (PLFA) analysis for profiling microbial communities in offshore produced water was optimized. The operation parameters affecting final PLFA profiling performance from the solid phase extraction (SPE) purification and fatty acid methyl esters (FAMEs) yielding process were investigated. Under the selected conditions, 92.9%, 96.3% and 92.8% of the spiked phospholipid standards C16:1 (cis-9) PC, C18:1 (cis-9) PC, and C19:0 PC were recovered, respectively, using 10mL methanol as elution solvent on a non-commercial SPE column. Over 90% of spiked C19:0 PC was recovered before sample transesterification. Four parameters including alkaline reagent, volume of acid for neutralization, time and temperature for FAMEs derivatization were examined. Gas Chromatography-Mass Spectrometry (GC-MS) was used to analyze FAMEs and the method linearities, recoveries of 29 FAMEs during transesterification, detection limits, relative standard deviations were presented. The results provided valuable information for biological reservoir souring control.
一种基于磷脂脂肪酸（PLFA）分析的用于近海采出水中微生物群落分析的方法得到了优化。研究了固相萃取（SPE）纯化和脂肪酸甲酯（FAMEs）生成过程中影响最终PLFA分析性能的操作参数。在选定条件下，使用10mL甲醇作为洗脱溶剂，在非商用SPE柱上，加标的磷脂标准品C16:1（顺式-9）PC、C18:1（顺式-9）PC和C19:0 PC的回收率分别为92.9%、96.3%和92.8%。在样品酯交换之前，加标的C19:0 PC的回收率超过90%。考察了包括碱性试剂、中和用酸的体积、FAMEs衍生化的时间和温度在内的四个参数。采用气相色谱-质谱联用（GC-MS）分析FAMEs，并给出了方法线性、酯交换过程中29种FAMEs的回收率、检测限、相对标准偏差。研究结果为生物储层酸化控制提供了有价值的信息。
