通过一种新型多重实时等位基因鉴别检测法快速鉴定ST131大肠杆菌
Rapid identification of ST131 Escherichia coli by a novel multiplex real-time allelic discrimination assay.
作者信息
François Patrice, Bonetti Eve-Julie, Fankhauser Carolina, Baud Damien, Cherkaoui Abdessalam, Schrenzel Jacques, Harbarth Stephan
机构信息
Genomic Research Laboratory, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland; Bacteriology Laboratory, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland.
Genomic Research Laboratory, University of Geneva Hospitals and Faculty of Medicine, Geneva, Switzerland.
出版信息
J Microbiol Methods. 2017 Sep;140:12-14. doi: 10.1016/j.mimet.2017.06.018. Epub 2017 Jun 26.
Escherichia coli sequence type 131 is increasingly described in severe hospital infections. We developed a rapid real-time allelic discrimination assay for the rapid identification of E. coli ST131 isolates. This rapid assay represents an affordable alternative to sequence-based strategies before completing characterization of potentially highly virulent isolates of E. coli.
131型大肠杆菌在严重的医院感染中越来越常见。我们开发了一种快速实时等位基因鉴别检测方法，用于快速鉴定131型大肠杆菌分离株。在完成对潜在高毒力大肠杆菌分离株的特征描述之前，这种快速检测方法是基于测序策略的一种经济实惠的替代方法。
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