Bai Lu, Huo Beibei, Chen Zhiqiang, Guo Qian, Xu Jing, Fang Jing, Zhang Jianghua, Zhang Fenfang
Hebei Medical University, Shijiazhuang, Hebei 050017, China.
Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China.
Evid Based Complement Alternat Med. 2017;2017:3054145. doi: 10.1155/2017/3054145. Epub 2017 Jun 1.
The study investigated the expression of Wnt/-catenin pathway in diabetic rats and the intervention effect of Huayu Tongluo herbs (HTH). Ten rats were randomly selected as control group and the remaining rats were established as diabetic models. The diabetic rats were randomly divided into model group and HTH treatment group. The intervention was intragastric administration in all rats for 20 weeks. At the end of every 4 weeks, fasting blood glucose and 24 h urinary total protein quantitatively were measured. At the end of the 20th week, biochemical parameters and body weight were tested. The kidney tissues were observed under light microscope and transmission electron microscopy. We examined Wnt/beta-catenin signaling pathway key proteins and renal interstitial fibrosis related molecular markers expression. The results showed that HTH could reduce urinary protein excretion and relieve renal pathological damage. Wnt4, p-GSK3 (S9), and -catenin expression were decreased in the signaling pathway, but GSK3 level was not changed by HTH in diabetic rats. Furthermore, the expressions of TGF- and ILK were decreased, but the level of E-cadherin was increased in diabetic rats after treatment with HTH. This study demonstrated that HTH could inhibit the high expression of Wnt/-catenin pathway in kidney of diabetic rats. The effect might be one of the main ways to reduce urinary protein excretion.
本研究探讨了Wnt/β-连环蛋白通路在糖尿病大鼠中的表达以及化瘀通络中药（HTH）的干预作用。随机选取10只大鼠作为对照组，其余大鼠建立糖尿病模型。将糖尿病大鼠随机分为模型组和HTH治疗组。所有大鼠均灌胃给药20周。每4周结束时，测定空腹血糖和24小时尿总蛋白定量。在第20周结束时，检测生化参数和体重。在光学显微镜和透射电子显微镜下观察肾组织。检测Wnt/β-连环蛋白信号通路关键蛋白及肾间质纤维化相关分子标志物的表达。结果显示，HTH可减少尿蛋白排泄，减轻肾脏病理损伤。信号通路中Wnt4、p-GSK3（S9）和β-连环蛋白表达降低，但HTH对糖尿病大鼠的GSK3水平无影响。此外，HTH治疗后糖尿病大鼠TGF-β和ILK的表达降低，但E-钙黏蛋白水平升高。本研究表明，HTH可抑制糖尿病大鼠肾脏中Wnt/β-连环蛋白通路的高表达。该作用可能是减少尿蛋白排泄的主要途径之一。
