Almani Suhail Ahmed, Memon Iqbal Ahmed, Shaikh Tariq Zaffar, Khoharo Haji Khan, Ujjan Ikramuddin
Department of Medicine Liaquat University of Medical and Health Sciences Jamshoro, Sindh, Pakistan.
Faculty of Medicine and Allied Medical Sciences Isra University, Hyderabad, Sindh, Pakistan.
Iran J Basic Med Sci. 2017 May;20(5):511-515. doi: 10.22038/IJBMS.2017.8675.
Causality of occurrence of metformin-associated lactic acidosis (MALA) is a clinical problem. Currently, there is no drug available to prevent MALA. The present study was conducted to evaluate the protective effect of Berberine (BBR) against MALA in induced diabetic rat model.
A sample of 75 healthy male Wistar rats was randomly selected according to inclusion and exclusion criteria. 75 male Wistar rats were randomly divided into a control and 4 experimental groups. Streptozotocin (STZ) in citrate buffer (pH 4.5) at a dose of 45 mg/kg was injected for induction of diabetes mellitus and rats achieving fasting blood glucose >250 mg/dl were included. Blood samples were collected 18 hr after the last dose of metformin and berberine. Ethical approval was taken before the study was conducted. Staistix 10.0 (USA) software was used for data analysis.
Berberine decreased MALA. Metformin, metformin + BBR 50 mg/kg bwt, and metformin + BBR 100 mg/kg bwt showed serum lactate as 1.87±0.4 mmol/lL, 1.62 ± 0.44 mmol/l and 1.47± 0.45 mmol/l, respectively (=0.0001). Insulin resistance and liver enzymes were improved in BBR treated rats.
The present study reports berberine protects against MALA in streptozocin-induced diabetes mellitus.
二甲双胍相关乳酸酸中毒（MALA）的发生原因是一个临床问题。目前，尚无预防MALA的药物。本研究旨在评估小檗碱（BBR）对诱导性糖尿病大鼠模型中MALA的保护作用。
根据纳入和排除标准，随机选取75只健康雄性Wistar大鼠。将75只雄性Wistar大鼠随机分为对照组和4个实验组。注射剂量为45mg/kg的柠檬酸盐缓冲液（pH 4.5）中的链脲佐菌素（STZ）以诱导糖尿病，纳入空腹血糖>250mg/dl的大鼠。在最后一次给予二甲双胍和小檗碱18小时后采集血样。在进行研究前获得了伦理批准。使用Staistix 10.0（美国）软件进行数据分析。
小檗碱可降低MALA。二甲双胍、二甲双胍+BBR 50mg/kg体重和二甲双胍+BBR 100mg/kg体重组的血清乳酸分别为1.87±0.4mmol/L、1.62±0.44mmol/L和1.47±0.45mmol/L（P=0.0001）。BBR治疗的大鼠胰岛素抵抗和肝酶得到改善。
本研究报告小檗碱对链脲佐菌素诱导的糖尿病中的MALA具有保护作用。
