Rahimi-Madiseh Mohammad, Karimian Paridokht, Kafeshani Marzieh, Rafieian-Kopaei Mahmoud
Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Department of Pathology, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Iran J Basic Med Sci. 2017 May;20(5):552-556. doi: 10.22038/IJBMS.2017.8681.
Various studies have shown that the diabetes is associated with liver failure. The objective of this study was determining the effects of fruit on histopathological and biochemical markers of liver in diabetic rats.
In this experimental study, 60 male Wistar rats weighing 200-250 g with free access to water and were randomly divided to five twelve-membered groups including healthy control (group 1), diabetic control (group 2, this two groups received distilled water), treated diabetic positive control (group 3) using dose 150 mg/kg/day metformin, and two groups treated with doses 200 (group 4) and 600 (group 5) mg/kg/BW of extracts via gavage feeding for 8 weeks. Diabetes mellitus was experimentally induced by one dose injection of alloxan 120 mg/kg. This pre-clinical study was performed on 120 mg/kg alloxan induced diabetic rats.
The hepatic steatosis status, liver cholestasis and fibrosis were not changed in group 4 and 5. Glycogen deposition changed mildly and polymorphonuclear neutrophils infiltration changed moderately at group 5. Liver hepatitis changed mildly and severely at group 3 as well as group 5, respectively. Glucose, serum glutamic-oxaloacetic transaminase, serum glutamic pyruvic transaminase, and alkaline phosphatase were lower in high dose group compared to other groups.
Results suggested that extract can decrease liver damage by influencing hepatic histopathological and biochemical markers in diabetic rats.
多项研究表明糖尿病与肝衰竭有关。本研究的目的是确定水果对糖尿病大鼠肝脏组织病理学和生化指标的影响。
在本实验研究中，将60只体重200 - 250克、可自由饮水的雄性Wistar大鼠随机分为五个每组12只的组，包括健康对照组（第1组）、糖尿病对照组（第2组，这两组给予蒸馏水）、使用150毫克/千克/天二甲双胍的糖尿病阳性治疗对照组（第3组），以及通过灌胃给予200毫克/千克体重（第4组）和600毫克/千克体重（第5组）提取物的两组，持续8周。通过一次性注射120毫克/千克四氧嘧啶实验性诱导糖尿病。本临床前研究是在120毫克/千克四氧嘧啶诱导的糖尿病大鼠上进行的。
第4组和第5组的肝脂肪变性状态、肝胆汁淤积和纤维化未发生变化。第5组糖原沉积轻度改变，多形核中性粒细胞浸润中度改变。第3组和第5组分别轻度和重度发生肝炎症改变。高剂量组的血糖、血清谷草转氨酶、血清谷丙转氨酶和碱性磷酸酶低于其他组。
结果表明提取物可通过影响糖尿病大鼠肝脏组织病理学和生化指标来减轻肝损伤。
