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通过深度测序对宫颈鳞癌患者的癌组织和正常组织进行转录组分析。

Transcriptome profiling of cancer and normal tissues from cervical squamous cancer patients by deep sequencing.

作者信息

Lin Wansong, Feng Mei, Li Xiuhua, Zhong Peilin, Guo Aihua, Chen Guilin, Xu Qin, Ye Yunbin

机构信息

Laboratory of Immuno‑Oncology, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.

Department of Gynecologic Oncology, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian 350014, P.R. China.

出版信息

Mol Med Rep. 2017 Aug;16(2):2075-2088. doi: 10.3892/mmr.2017.6855. Epub 2017 Jun 23.

DOI:10.3892/mmr.2017.6855
PMID:28656315
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5562054/
Abstract

Cervical cancer is the fourth leading cause of cancer mortality in women worldwide. High‑risk human papillomavirus infection is a major cause of cervical cancer. A previous study revealed the role of different oncogenes and tumor suppressors in cervical cancer initiation and progression. However, the complicated genetic network regulating cervical cancer remains largely unknown. The present study reported transcriptome sequencing analysis of three cervical squamous cell cancer tissues and paired normal cervical tissues. Transcriptomic analysis revealed that 2,519 genes were differently expressed between cervical cancer tissues and their corresponding normal tissues. Among these, 236 differentially expressed genes (DEGs) were statistically significant, including many DEGs that were novel in cervical cancer, including gastrulation brain homeobox 2,5‑hydroxytryptamine receptor 1D and endothelin 3. These 236 significant DEGs were highly enriched in 28 functional gene ontology categories. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis suggested involvement of these DEGs in multiple pathways. The present study provides a transcriptome landscape of cervical cancer in Chinese patients and an improved understanding of the genetic regulatory network in cervical cancer tumorigenesis.

摘要

宫颈癌是全球女性癌症死亡的第四大主要原因。高危型人乳头瘤病毒感染是宫颈癌的主要病因。先前的一项研究揭示了不同癌基因和肿瘤抑制因子在宫颈癌发生和发展中的作用。然而，调节宫颈癌的复杂基因网络在很大程度上仍然未知。本研究报告了对三个宫颈鳞状细胞癌组织和配对的正常宫颈组织的转录组测序分析。转录组分析显示，宫颈癌组织与其相应正常组织之间有2519个基因表达存在差异。其中，236个差异表达基因（DEG）具有统计学意义，包括许多在宫颈癌中首次发现的DEG，如原肠胚形成脑同源框2、5-羟色胺受体1D和内皮素3。这236个显著的DEG在28个功能基因本体类别中高度富集。京都基因与基因组百科全书通路富集分析表明这些DEG参与了多个通路。本研究提供了中国患者宫颈癌的转录组图谱，并增进了对宫颈癌肿瘤发生中基因调控网络的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6678/5562054/a00f759671cf/MMR-16-02-2075-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6678/5562054/4deddaa47a24/MMR-16-02-2075-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6678/5562054/30e089e034cc/MMR-16-02-2075-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6678/5562054/a00f759671cf/MMR-16-02-2075-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6678/5562054/4deddaa47a24/MMR-16-02-2075-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6678/5562054/30e089e034cc/MMR-16-02-2075-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6678/5562054/a00f759671cf/MMR-16-02-2075-g05.jpg

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