School of Municipal and Environmental Engineering, Harbin Institute of Technology , Harbin 150080, China.
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences , Harbin 150069, China.
ACS Appl Mater Interfaces. 2017 Jul 19;9(28):23450-23457. doi: 10.1021/acsami.7b05867. Epub 2017 Jul 5.
A bimodal contrast nanoagent was developed by chelating gadolinium ions to 2-[bis[2-[carboxymethyl-[2-oxo-2-(2-sulfanylethyl-amino)ethyl]amino]ethyl]amino]acetic acid (DTDTPA)-modified CuInS/ZnS quantum dots (QDs). The longitudinal relaxivity (r) of the resulted QDs@DTDTPA-Gd nanoparticles (NPs) was calculated to be 9.91 mM s, which was 2.5 times as high as that of clinically approved Gd-DTPA (3.9 mM s). In addition, the in vivo imaging experiments showed that QDs@DTDTPA-Gd NPs could enhance both near-infrared fluorescence and T-weighted magnetic resonance (MR) imaging of tumor tissue through passive targeting accumulation. Moreover, the high colloidal and fluorescence stabilities and good biocompatibility indicate that QDs@DTDTPA-Gd NPs have a great potential for use as an efficient nanoagent to integrate the extremely high sensitivity of fluorescence imaging to the high resolution of MR imaging. Integration of bimodal detectability in the same agent of QDs@DTDTPA-Gd NPs can avoid extra stress on the blood clearance mechanisms as the administration of multiple dose of agents.
一种双模态对比纳米试剂通过螯合钆离子到 2-[双[2-[羧甲基-[2-硫代乙基-氨基]乙基]氨基]乙基]氨基]乙酸（DTDTPA）-修饰的 CuInS/ZnS 量子点（QDs）来制备。所得 QDs@DTDTPA-Gd 纳米颗粒（NPs）的纵向弛豫率（r）计算为 9.91 mM s，是临床批准的 Gd-DTPA（3.9 mM s）的 2.5 倍。此外，体内成像实验表明，QDs@DTDTPA-Gd NPs 可以通过被动靶向积累增强肿瘤组织的近红外荧光和 T 加权磁共振（MR）成像。此外，高胶体和荧光稳定性以及良好的生物相容性表明，QDs@DTDTPA-Gd NPs 具有作为高效纳米试剂的巨大潜力，可将荧光成像的超高灵敏度与 MR 成像的高分辨率集成在同一试剂中。在 QDs@DTDTPA-Gd NPs 中整合双模态检测能力可以避免由于给予多个剂量的试剂而对血液清除机制造成额外的压力。
