Albrecht Claudia, Guzmán María Laura, Cittadini María Cecilia, Canalis Alejandra Mariel, Valentich Mirta Ana, Olivera María Eugenia, Soria Elio Andrés
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Rev Fac Cien Med Univ Nac Cordoba. 2017 Dec 21;74(4):338-344. doi: 10.31053/1853.0605.v74.n4.15412.
Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment of different illnesses. This also supports the intake of teas as functional beverages. Nonetheless, it is incompletely known whether these extracts can act as effective sources and vehicles de phenolic compounds (phenolics/polyphenols) to reach their targets.
To establish whether phytoextract ingestion modified in a sex-dependent manner the phenolic bioavailability and redox response in liver and kidney.
BALB/C mice ingested for a month 100 mg/Kg/d of extracts (tea-like) from Aspidosperma quebracho-blanco (AQB), Lantana grisebachii (LG) or Ilex paraguariensis (IP). Then, phenolics, peroxides and nitrites were analyzed by spectrophotometry. Also, phenolic permeation from digested and undigested extracts was evaluated in vitro with a rat jejunum-based assay.
Phenolic permeation depended on extract digestion. In males, IP showed a special time course of hepatic phenolics, whereas all extracts decreased renal phenolics at 15 days. Extracts induced hepatic lipoperoxides at 15 days. LG reduced renal hydroperoxides at 15 days and hepatic nitrites at 30 days, whereas AQB and IP reduced renal lipoperoxides and nitrites at 30 days. In females, extracts reduced hydroperoxides, with LG and AQB also reducing lipoperoxides. IP increased renal lipoperoxides at 30 days.
IP was a relevant phenolic source. Sex-dependent responses were found in all variables, which should be considered to prevent misleading generalizations in phytodrug bioprospecting.
可获取植物提取物以携带生物活性化合物，这些化合物对预防和治疗不同疾病有用。这也支持将茶作为功能性饮料饮用。然而，这些提取物是否能作为酚类化合物（酚类/多酚）的有效来源和载体以到达其靶点尚不完全清楚。
确定摄入植物提取物是否会以性别依赖的方式改变肝脏和肾脏中酚类物质的生物利用度和氧化还原反应。
BALB/C小鼠连续一个月每天按100mg/Kg的剂量摄入来自白坚木（AQB）、灰莉（LG）或巴拉圭冬青（IP）的提取物（类茶）。然后，通过分光光度法分析酚类物质、过氧化物和亚硝酸盐。此外，采用基于大鼠空肠的试验在体外评估消化和未消化提取物中酚类物质的渗透情况。
酚类物质的渗透取决于提取物的消化情况。在雄性小鼠中，IP显示出肝脏酚类物质的特殊时间进程，而所有提取物在15天时均降低了肾脏中的酚类物质。提取物在15天时诱导肝脏产生脂质过氧化物。LG在15天时降低了肾脏中的氢过氧化物，在30天时降低了肝脏中的亚硝酸盐，而AQB和IP在30天时降低了肾脏中的脂质过氧化物和亚硝酸盐。在雌性小鼠中，提取物降低了氢过氧化物，LG和AQB还降低了脂质过氧化物。IP在30天时增加了肾脏中的脂质过氧化物。
IP是一种重要的酚类来源。在所有变量中均发现了性别依赖性反应，在植物药生物勘探中应考虑这些反应以避免产生误导性的一般性结论。
