Lingabathula Harikiran, Yellu Narsimhareddy
Department of Pharmacology and Toxicology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, India.
Drug Res (Stuttg). 2017 Oct;67(10):606-612. doi: 10.1055/s-0043-113255. Epub 2017 Jun 28.
The gold nanorods (GNRs) and silver nanorods (SNRs) are utilized in various types of industrial and commercial applications. But, there is limited availability of extra pulmonary toxicity data regarding these nanorods. The present investigation evaluated the extra pulmonary toxicity induced by 10 and 25 nm GNRs and SNRs in rats following intra tracheal instillation. The serum biochemical analysis results have shown elevated levels of serum alanine transaminase (ALT) and serum creatinine following 1 day and 1 week post instillation. GNRs have shown greatly increased serum ALT levels at 1 day, 1 week and 1 month post exposure periods compared to SNRs and quartz (QTZ) treated rats. In case of serum creatinine levels, both GNRs and SNRs have shown similar elevated levels. Histopathology studies of rat liver tissues following exposure of GNRs and SNRs displayed that congestion of central vein, shrinkage and ballooning of hepatocytes and lymphocytic infiltration leading to degeneration after 1 week and 1 month post instillation periods. The histopathology of rat kidney tissue was showed tubular dilation, degeneration and necrosis with 10 nm SNRs and 10 nm GNRs after 1 month post instillation period. The 10 nm GNRs and SNRs have shown great changes in serum biochemical analysis and histopathological studies compared to 25 nm test nanorods. These observations suggest the size and dose dependent translocation and extra pulmonary toxicity of both GNRs and SNRs.
金纳米棒（GNRs）和银纳米棒（SNRs）被用于各种工业和商业应用。但是，关于这些纳米棒的肺外毒性数据有限。本研究评估了经气管内滴注后，10纳米和25纳米的GNRs和SNRs在大鼠体内诱导的肺外毒性。血清生化分析结果显示，滴注后1天和1周，血清丙氨酸转氨酶（ALT）和血清肌酐水平升高。与SNRs和石英（QTZ）处理的大鼠相比，GNRs在暴露后1天、1周和1个月时血清ALT水平大幅升高。就血清肌酐水平而言，GNRs和SNRs均显示出相似的升高水平。暴露于GNRs和SNRs后的大鼠肝组织病理研究显示，滴注后1周和1个月，中央静脉充血、肝细胞萎缩和气球样变以及淋巴细胞浸润导致变性。滴注后1个月，10纳米的SNRs和10纳米的GNRs使大鼠肾组织病理显示肾小管扩张、变性和坏死。与25纳米的测试纳米棒相比，10纳米的GNRs和SNRs在血清生化分析和组织病理学研究中显示出巨大变化。这些观察结果表明GNRs和SNRs的大小和剂量依赖性易位及肺外毒性。
