Catalan Institute for Water Research (ICRA), H2O Building, Scientific and Technological Park of the University of Girona, Emili Grahit 101, 17003, Girona, Spain; Water and Soil Quality Research Group, Department of Environmental Chemistry, IDAEA-CSIC, Jordi Girona 18-26, 08034, Barcelona, Spain.
Departament d'Enginyeria Química, Escola d'Enginyeria, Universitat Autònoma de Barcelona (UAB), 08193, Bellaterra, Spain.
Chemosphere. 2017 Oct;184:1054-1070. doi: 10.1016/j.chemosphere.2017.06.053. Epub 2017 Jun 15.
The removal of 27 endocrine-disrupting compounds and related compounds (suspect effect) from a reverse osmosis concentrate using an alternative decontamination method based on a fungal treatment involving Trametes versicolor was assessed. In addition to chemical analysis, the toxicity of the treated water during the treatment was monitored using a bioluminescence inhibition test and estrogenic and anti-estrogenic tests. The compounds 1H-benzotriazole (BTZ) and two tolyltriazoles (TTZs), 4-methyl-1H-benzotriazole (4-MBTZ) and 5-methyl-1H-benzotriazole (5-MBTZ), were present in the reverse osmosis concentrate at the highest concentrations (7.4 and 12.8 μg L, respectively) and were partially removed by the fungal treatment under sterile conditions (58% for BTZ and 92% for TTZs) and non-sterile conditions, although to lesser extents (32% for BTZ and 50% for TTZs). Individual biotransformation studies of BTZ and the TTZs by T. versicolor in a synthetic medium and further analysis via on-line turbulent flow chromatography coupled to an HRMS-Orbitrap allowed the tentative identification of the transformation products (TPs). Six TPs were postulated for BTZ, two TPs were postulated for 4-MBTZ, and four TPs were postulated for 5-MBTZ. Most of these TPs are suggested to have been generated by conjugation with some sugars and via the methylation of the triazole group. Only TP 148 A, postulated to be derived from the biotransformation of BTZ, was observed in the effluent of the bioreactor treating the reverse osmosis concentrate.
采用基于涉及彩绒革盖菌（Trametes versicolor）的真菌处理的替代净化方法，从反渗透浓缩物中去除 27 种内分泌干扰化合物和相关化合物（可疑效应），并对其进行了评估。除了化学分析外，还使用生物发光抑制试验以及雌激素和抗雌激素试验监测处理过程中处理水的毒性。在反渗透浓缩物中，1H-苯并三唑（BTZ）和两种甲苯三唑（TTZs）、4-甲基-1H-苯并三唑（4-MBTZ）和 5-甲基-1H-苯并三唑（5-MBTZ）的浓度最高（分别为 7.4 和 12.8μg/L），真菌处理在无菌条件下（BTZ 为 58%，TTZs 为 92%）和非无菌条件下部分去除了这些化合物，但去除程度较低（BTZ 为 32%，TTZs 为 50%）。彩绒革盖菌在合成培养基中对 BTZ 和 TTZs 进行的个体生物转化研究，以及通过在线紊流色谱与 HRMS-Orbitrap 进一步分析，允许对转化产物（TPs）进行推测性鉴定。推测 BTZ 有 6 种 TPs，4-MBTZ 有 2 种 TPs，5-MBTZ 有 4 种 TPs。这些 TPs 中的大多数据推测是通过与某些糖的结合以及三唑基团的甲基化而生成的。只有推测源自 BTZ 生物转化的 TP 148 A 在处理反渗透浓缩物的生物反应器的流出物中被观察到。
