Pawashe Karuna Gajanan, Tewary Shivsagar, Sanyal Pronob Kumar, Nilesh Kumar
Assistant Professor, Department of Prosthodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed University, Malkapur, Karad, Maharashtra, India.
Reader, Department of Prosthodontics, School of Dental Sciences, Krishna Institute of Medical Sciences Deemed University, Malkapur, Karad, Maharashtra, India.
J Clin Diagn Res. 2017 May;11(5):ZC54-ZC58. doi: 10.7860/JCDR/2017/24759.9866. Epub 2017 May 1.
Oral cavity is colonised by numerous micro-organisms that form a biofilm on the acrylic resin. Hence, routine hygiene is essential to prevent oral mucosal inflammation and lesions. Knowledge of appropriate disinfecting agents for acrylic resins is crucial in this context.
To compare and evaluate the effectiveness of four commercially available disinfectants on heat cure acrylic resin specimens contaminated with standard and clinical strains of two micro-organisms commonly inhabiting the oral microflora.
Two hundred acrylic resin specimens (n=200), 10 in each group were contaminated in vitro with 1x10 cells/ml suspensions of standard and clinical strains of micro-organisms and were immersed in four disinfectants (1% sodium hypochlorite, 2% chlorhexidine digluconate, 2% glutaraldehyde and 3.8% sodium perborate) for 10 minutes. The control group was not subjected to any disinfection process. For collection of clinical strains, oral swab was passed over the buccal mucosa and grown on blood agar culture media. Organism confirmation was done by growing them on selective culture media. Final counts of micro-organisms per ml were performed by plating method for evaluation of microbial level reduction. Results obtained were subjected to ANOVA and Tukey's test.
Standard strains of (C) and (S) subjected to various disinfectants showed varied mean Colony Forming Units per ml (CFU/ml) from <10,000 to 25,000 and <10000 to <50,000 respectively. Clinical strains and of (C) and (S) subjected to various disinfectants showed varied mean CFU/ml from <10,000 to 50,000 and from 10,000 to 50,000 respectively. Control groups showed maximum mean CFU/ml (>10). All intergroup comparisons were highly significant (p<0.001; HS) and intragroup comparisons were significant (p<0.05; S) except the comparison of clinical strains of subjected to 2% chlorhexidine digluconate and 2% glutaraldehyde which was found to be non significant (p>0.05; NS).
Almost 1% sodium hypochlorite was found to be the most effective disinfectant for both and . The least effective disinfectant being: 3.8% sodium perborate.
口腔中定植着众多微生物，它们在丙烯酸树脂上形成生物膜。因此，日常口腔卫生对于预防口腔黏膜炎症和病变至关重要。在这种情况下，了解适用于丙烯酸树脂的消毒剂至关重要。
比较和评估四种市售消毒剂对被口腔微生物群落中两种常见微生物的标准菌株和临床菌株污染的热固化丙烯酸树脂标本的有效性。
200个丙烯酸树脂标本（n = 200），每组10个，在体外被微生物标准菌株和临床菌株的1x10细胞/ml悬浮液污染，并浸泡在四种消毒剂（1%次氯酸钠、2%葡萄糖酸洗必泰、2%戊二醛和3.8%过硼酸钠）中10分钟。对照组未进行任何消毒处理。为收集临床菌株，将口腔拭子在颊黏膜上擦拭并接种于血琼脂培养基上培养。通过在选择性培养基上培养进行微生物鉴定。采用平板计数法进行每毫升微生物的最终计数，以评估微生物数量的减少情况。对所得结果进行方差分析和Tukey检验。
受到各种消毒剂处理的（C）和（S）标准菌株每毫升的平均菌落形成单位（CFU/ml）各不相同，分别为<10,000至25,000以及<10,000至<50,000。受到各种消毒剂处理的（C）和（S）临床菌株每毫升的平均CFU/ml分别为<10,000至50,000以及10,000至50,000。对照组显示出最高的平均CFU/ml（>10）。除了对受到2%葡萄糖酸洗必泰和2%戊二醛处理的（）临床菌株的比较发现无显著差异（p>0.05；无显著性）外，所有组间比较均具有高度显著性（p<0.001；高度显著性），组内比较具有显著性（p<0.05；显著性）。
发现几乎1%的次氯酸钠对（）和（）都是最有效的消毒剂。最无效的消毒剂是：3.8%过硼酸钠。
