Kearns M, Lala P K
Am J Reprod Immunol Microbiol. 1985 Oct;9(2):39-47. doi: 10.1111/j.1600-0897.1985.tb00273.x.
Stromal type decidual cells recovered from the murine decidua by a mild collagenase dispersion procedure contain immunoregulatory cells whose ultimate precursors may originate from the bone marrow. To explore the familial relationship of these cells with other cells of the immune system, a battery of cell surface markers recognized on lymphomyeloid cells were examined and quantitated at the morphological level on typical stromal type decidual cells of the dispersed CBA mouse decidua at 8-14 days of syngeneic pregnancy, using a sensitive radioautographic technique. Cells were either labeled directly by exposure to 125I-labeled monoclonal antibodies against Thy-1, Mac-1, or Lyt antigens or indirectly by a sequential exposure to monoclonal anti-I-Ak (Ia.17) or monospecific anti-I-JK antibodies and 125I-labeled Protein A. Decidual cells were found to be Thy-1+/- (13-73% positive, the incidence rising with advancing gestation in the decidua basalis), Mac-1+/- (present of 6-11% on day 8 and 17-32% on day 12), I-A-, 1-J-, and Lyt-. Macrophages within the decidua were Thy-1-, Lyt-, Mac-1+, and I-A+/- (present on 5-61% of cells, the incidence rising with advancing gestation).(ABSTRACT TRUNCATED AT 250 WORDS)
通过温和的胶原酶分散程序从小鼠蜕膜中回收的基质型蜕膜细胞含有免疫调节细胞，其最终前体可能起源于骨髓。为了探究这些细胞与免疫系统其他细胞的家族关系，使用灵敏的放射自显影技术，在形态学水平上对同种异体妊娠8至14天的CBA小鼠蜕膜典型基质型蜕膜细胞上识别的一系列淋巴细胞表面标志物进行了检测和定量。细胞要么通过暴露于针对Thy-1、Mac-1或Lyt抗原的125I标记单克隆抗体直接标记，要么通过依次暴露于单克隆抗I-Ak（Ia.17）或单特异性抗I-JK抗体以及125I标记的蛋白A间接标记。发现蜕膜细胞为Thy-1+/-（13 - 73%阳性，在基蜕膜中随着妊娠进展发生率升高），Mac-1+/-（第8天为6 - 11%，第12天为17 - 32%），I-A-，I-J-，以及Lyt-。蜕膜内的巨噬细胞为Thy-1-，Lyt-，Mac-1+，以及I-A+/-（存在于5 - 61%的细胞中，随着妊娠进展发生率升高）。（摘要截短于250字）
