Sánchez Manuel, Suárez Lorena, Andrés María Teresa, Flórez Blanca Henar, Bordallo Javier, Riestra Sabino, Cantabrana Begoña
Farmacología, Departamento de Medicina, Universidad de Oviedo, Oviedo, Spain.
Instituto Universitario de Oncología del Principado de Asturias (IUOPA), Oviedo, Spain.
Food Nutr Res. 2017 May 26;61(1):1321948. doi: 10.1080/16546628.2017.1321948. eCollection 2017.
: Gastrointestinal motility modulatory factors include substances of the intestinal content, such as polyamines and trace amines (TAs), the focus of this study. : The amines of food, intestinal content and from faecal bacteria of Swiss mice were determined by HPLC and functionally characterised in isolated distal ileum and medial colon rings. : Mouse food and intestinal content contain polyamines (spermidine>putrescine>spermine) and TAs (isoamylamine>cadaverine). Intestinal bacteria mainly produce putrescine and cadaverine. The amines inhibited the spontaneous motility of the ileum (0.1-3 mM) and colon rings (0.01-3 mM, with lower IC), with: spermineisoamylaminespermidine. Spermine inhibition was tetrodotoxin (TTX)-insensitive, while isoamylamine was TTX-sensitive, suggesting neural control. Mainly in the ileum, isoamylamine (3 mM) elicited acute effects modified by TTX, atropine and propranolol, and suppressed by spermine (3 mM), not being localized at the smooth muscle level. The amines assayed (3 mM), except putrescine and cadaverine in the ileum and isoamylamine in the colon, antagonised acetylcholine (ACh, 0.1 mM)-elicited phasic contractions. Isoamylamine and spermine in colon relaxed KCl (100 mM)-elicited tonic contractions, suggesting an effect on smooth muscle, but did not justify the suppression of motility caused by spermine and isoamylamine. : Polyamines and TAs of the intestinal content might act on chemosensors and modulate intestinal peristalsis.
胃肠道动力调节因子包括肠内容物中的物质，如多胺和微量胺（TAs），本研究的重点即在于此。
通过高效液相色谱法测定了瑞士小鼠食物、肠内容物及粪便细菌中的胺，并在离体的回肠末端和结肠中环进行了功能特性分析。
小鼠食物和肠内容物中含有多胺（亚精胺>腐胺>精胺）和微量胺（异戊胺>尸胺）。肠道细菌主要产生腐胺和尸胺。这些胺抑制回肠（0.1 - 3 mM）和结肠环（0.01 - 3 mM，抑制浓度较低）的自发运动，抑制效果为：精胺异戊胺亚精胺。精胺的抑制作用对河豚毒素（TTX）不敏感，而异戊胺对TTX敏感，提示存在神经控制。主要在回肠中，异戊胺（3 mM）引发的急性效应可被TTX、阿托品和普萘洛尔改变，并被精胺（3 mM）抑制，且并非定位于平滑肌水平。所检测的胺（3 mM），除了回肠中的腐胺和尸胺以及结肠中的异戊胺外，均拮抗乙酰胆碱（ACh，0.1 mM）引发的相性收缩。结肠中的异戊胺和精胺可舒张氯化钾（100 mM）引发的强直性收缩，提示对平滑肌有作用，但无法解释精胺和异戊胺对运动的抑制作用。
肠内容物中的多胺和微量胺可能作用于化学感受器并调节肠道蠕动。
