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葡萄球菌对多胺的代谢

Metabolism of polyamines by Staphylococcus.

作者信息

ROSENTHAL S M, DUBIN D T

出版信息

J Bacteriol. 1962 Oct;84(4):859-63. doi: 10.1128/jb.84.4.859-863.1962.

DOI:10.1128/jb.84.4.859-863.1962
PMID:13974996
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC277968/
Abstract

Rosenthal, Sanford M. (National Institute of Arthritis and Metabolic Diseases, Bethesda, Md.) and Donald T. Dubin. Metabolism of polyamines by Staphylococcus. J. Bacteriol. 84:859-863. 1962-Although spermine and spermidine are not detectable in staphylococci grown in purified medium, considerable amounts (0.7 to 1.4 mumole/g wet wt) were demonstrated in cells grown in the presence of these amines. Conjugation of spermine, and to a lesser extent of spermidine, was demonstrated. Putrescine and cadaverine added to purified medium are not appreciably taken up by staphylococcal cells. However, high percentages were recovered in the medium in conjugated form. These conjugates were shown to be monoacetylputrescine and probably monoacetylcadaverine. Meat infusion broth, neopeptone, spermine, and spermidine were highly inhibitory to this acetylation. No evidence of utilization of these polyamines was obtained.

摘要

罗森塔尔,桑福德·M.(国立关节炎与代谢疾病研究所,马里兰州贝塞斯达)和唐纳德·T. 杜宾。葡萄球菌对多胺的代谢。《细菌学杂志》84:859 - 863。1962年——尽管在纯化培养基中生长的葡萄球菌中检测不到精胺和亚精胺,但在这些胺存在的情况下生长的细胞中显示出相当数量(0.7至1.4微摩尔/克湿重)。已证实精胺以及程度较轻的亚精胺发生了共轭作用。添加到纯化培养基中的腐胺和尸胺未被葡萄球菌细胞明显摄取。然而,以共轭形式在培养基中回收的比例很高。这些共轭物被证明是单乙酰腐胺,可能还有单乙酰尸胺。肉浸液肉汤、新蛋白胨、精胺和亚精胺对这种乙酰化具有高度抑制作用。未获得利用这些多胺的证据。

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Inhibition of protein synthesis by spermine in growing cells of Staphylococcus aureus.精胺对金黄色葡萄球菌生长细胞中蛋白质合成的抑制作用。
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本文引用的文献

1
The acetylation of polyamines in Escherichia coli.大肠杆菌中多胺的乙酰化作用。
J Biol Chem. 1960 Mar;235:776-82.
2
The biosynthesis of spermidine and spermine from putrescine and methionine.由腐胺和蛋氨酸生物合成亚精胺和精胺。
J Biol Chem. 1958 Oct;233(4):907-14.
3
Metabolism of diamines and polyamines in microorganisms.微生物中二胺和多胺的代谢
J Biol Chem. 1958 Apr;231(2):637-46.
4
The gram reaction and cell composition: diamines and polyamines.革兰氏反应与细胞组成:二胺和多胺
Arch Biochem Biophys. 1958 May;75(1):171-7. doi: 10.1016/0003-9861(58)90407-7.
5
The pharmacology of spermine and spermidine; distribution and excretion.精胺和亚精胺的药理学;分布与排泄。
J Pharmacol Exp Ther. 1956 Feb;116(2):131-8.
6
Enzymatic phosphorylation of acetate.乙酸的酶促磷酸化作用。
J Biol Chem. 1954 Dec;211(2):737-56.