The presence of three neuroactive peptides in putative glycinergic amacrine cells of an avian retina.

Amacrine cells are retinal interneurons which serve to mediate transmission between bipolar and ganglion cells. To date, in addition to several classical neurotransmitters, a number of neuroactive peptides have been localized to these cells. We have previously demonstrated in the chicken that both peptide-transmitter and peptide-peptide colocalization exists in some amacrine cells. In this report, we show that 3 neuroactive peptides (enkephalin, neurotensin and somatostatin) are present in subpopulations of amacrine cells which also possess a high affinity uptake system for glycine. These observations suggest that the simultaneous visualization by autoradiography of [3H]glycine-uptake and immunocytochemistry of peptides may be useful for distinguishing between different types of putative glycinergic amacrine cells.