固态发酵染料生物修复后产生的废弃木质纤维素生物质的酶解及特性研究。
Enzymatic hydrolysis and characterization of waste lignocellulosic biomass produced after dye bioremediation under solid state fermentation.
机构信息
Department of Biochemistry, Shivaji University, Kolhapur 416004, India.
Department of Biotechnology, Shivaji University, Kolhapur 416004, India.
出版信息
Bioresour Technol. 2014 Sep;168:136-41. doi: 10.1016/j.biortech.2014.02.099. Epub 2014 Mar 4.
Sugarcane bagasse (SCB) adsorbes 60% Reactive Blue172 (RB172). Providensia staurti EbtSPG able to decolorize SCB adsorbed RB172 up to 99% under solid state fermentation (SSF). The enzymatic saccharification efficiency of waste biomass after bioremediation of RB172 process (ddSCB) has been evaluated. The cellulolyitc crude enzyme produced by Phanerochaete chrysosporium used for enzymatic hydrolysis of native SCB and ddSCB which produces 0.08 and 0.3 g/L of reducing sugars respectively after 48 h of incubation. The production of hexose and pentose sugars during hydrolysis was confirmed by HPTLC. The effect of enzymatic hydrolysis on SCB and ddSCB has been evaluated by FTIR, XRD and SEM analysis. Thus, during dye biodegradation under SSF causes biological pretreatment of SCB which significantly enhanced its enzymatic saccharification. Adsorption of dye on SCB, its bioremediation under SSF produces wastes biomass and which further utilized for enzymatic saccharification for biofuel production.
甘蔗渣（SCB）吸附 60%活性蓝 172（RB172）。Providenia staurti EbtSPG 能够在固态发酵（SSF）下将 SCB 吸附的 RB172 脱色高达 99%。对 RB172 处理过程中生物修复后的废生物质（ddSCB）的酶解糖化效率进行了评估。使用黄孢原毛平革菌产生的纤维素粗酶进行酶解，分别在 48 小时的孵育后产生 0.08 和 0.3 g/L 的还原糖。通过 HPTLC 确认了水解过程中六糖和五糖的生成。通过 FTIR、XRD 和 SEM 分析评估了酶解对 SCB 和 ddSCB 的影响。因此，在 SSF 下进行染料生物降解会导致 SCB 的生物预处理，从而显著提高其酶解糖化效率。染料在 SCB 上的吸附，以及 SSF 下的生物修复产生了废生物质，可进一步用于酶解糖化以生产生物燃料。
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