Shanghai Advanced Research Institute , Chinese Academy of Sciences, Shanghai 201210, China.
Shanghai University of Medicine & Health Sciences , Shanghai 201318, China.
ACS Appl Mater Interfaces. 2017 Jul 19;9(28):24118-24125. doi: 10.1021/acsami.7b05981. Epub 2017 Jul 5.
Specific and sensitive biomarker detection is essential to early cancer diagnosis. In this study, we demonstrate an ultrasensitive electrochemical biosensor with the ability to detect multiple pancreatic carcinoma (PC)-related microRNA biomarkers. By employing DNA tetrahedral nanostructure capture probes to enhance the detection sensitivity as well as a disposable 16-channel screen-printed gold electrode (SPGE) detection platform to enhance the detection efficiency, we were able to simultaneously detect four PC-related miRNAs: miRNA21, miRNA155, miRNA196a, and miRNA210. The detection sensitivity reached to as low as 10 fM. We then profiled the serum levels of the four miRNAs for PC patients and healthy individuals with our multiplexing electrochemical biosensor. Through the combined analyses of the four miRNAs, our results showed that PC patients could be discriminated from healthy controls with fairly high sensitivity. This multiplexing PCR-free miRNA detection sensor shows promising applications in early diagnosis of PC disease.
特异性和敏感性生物标志物检测对于癌症的早期诊断至关重要。在本研究中，我们展示了一种超灵敏的电化学生物传感器，能够检测多种胰腺癌（PC）相关 microRNA 生物标志物。通过使用 DNA 四面体型纳米结构捕获探针来提高检测灵敏度，以及采用一次性 16 通道丝网印刷金电极（SPGE）检测平台来提高检测效率，我们能够同时检测四种与 PC 相关的 microRNA：miRNA21、miRNA155、miRNA196a 和 miRNA210。检测灵敏度低至 10 fM。然后，我们使用我们的多路复用电化学生物传感器来分析 PC 患者和健康个体的四种 microRNA 的血清水平。通过对四种 microRNA 的综合分析，我们的结果表明，PC 患者与健康对照者相比，具有相当高的灵敏度。这种无需聚合酶链反应（PCR）的多路复用 microRNA 检测传感器在 PC 疾病的早期诊断中具有广阔的应用前景。
