Naranbat Dulguunnaran, Herdes Emilia, Tapinos Nikos, Tripathi Anubhav
Center for Biomedical Engineering, School of Engineering, Brown University, Providence, RI, USA.
Warren Alpert Medical School, Brown University, Providence, RI, USA.
Expert Rev Mol Med. 2025 Feb 6;27:e11. doi: 10.1017/erm.2025.2.
MicroRNAs have emerged as effective biomarkers in disease diagnostics, particularly cancer, due to their role as regulatory sequences. More recently, microRNAs have been detected in liquid biopsies, which hold immense potential for early disease diagnostics. This review comprehensively analyses distinct liquid biopsy microRNA detection methods validated with clinical samples. Each step in the microRNA detection workflow, including sample collection, RNA isolation, processing, and detection of target microRNAs, has been thoroughly assessed. The review discusses the advantages and limitations of established and novel techniques in microRNA detection workflows, discussing their diagnostic capabilities and potential for future implementation at scale.
由于其作为调控序列的作用，微小RNA已成为疾病诊断（尤其是癌症诊断）中的有效生物标志物。最近，在液体活检中检测到了微小RNA，这为疾病早期诊断具有巨大潜力。本综述全面分析了已用临床样本验证的不同液体活检微小RNA检测方法。微小RNA检测工作流程中的每一步，包括样本采集、RNA分离、处理以及目标微小RNA的检测，都已得到全面评估。该综述讨论了微小RNA检测工作流程中成熟技术和新技术的优点与局限性，探讨了它们的诊断能力以及未来大规模应用的潜力。
