Zou Shangjie, Peng Guangdun, Ma Zhiqiang
Center for Cell Lineage Technology and Engineering, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, GIBH-CUHK Joint Research Laboratory on Stem Cell and Regenerative Medicine, GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, China-New Zealand Belt and Road Joint Laboratory on Biomedicine and Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong 999077, China.
Nanomaterials (Basel). 2024 Dec 14;14(24):2014. doi: 10.3390/nano14242014.
Multiplexed biosensing methods for simultaneously detecting multiple biomolecules are important for investigating biological mechanisms associated with physiological processes, developing applications in life sciences, and conducting medical tests. The development of biosensors, especially those advanced biosensors with multiplexing potentials, strongly depends on advancements in nanotechnologies, including the nano-coating of thin films, micro-nano 3D structures, and nanotags for signal generation. Surface functionalization is a critical process for biosensing applications, one which enables the immobilization of biological probes or other structures that assist in the capturing of biomolecules. During this functionalizing process, nanomaterials can either be the objects of surface modification or the materials used to modify other base surfaces. These surface-functionalizing strategies, involving the coordination of sensor structures and materials, as well as the associated modifying methods, are largely determinative in the performance of biosensing applications. This review introduces the current studies on biosensors with multiplexing potentials and focuses specifically on the roles of nanomaterials in the design and functionalization of these biosensors. A detailed description of the paradigms used for method selection has been set forth to assist understanding and accelerate the application of novel nanotechnologies in the development of biosensors.
用于同时检测多种生物分子的多重生物传感方法对于研究与生理过程相关的生物学机制、开发生命科学应用以及进行医学检测具有重要意义。生物传感器的发展，尤其是那些具有多重检测潜力的先进生物传感器，在很大程度上依赖于纳米技术的进步，包括薄膜的纳米涂层、微纳三维结构以及用于信号产生的纳米标签。表面功能化是生物传感应用的关键过程，它能够固定生物探针或其他有助于捕获生物分子的结构。在这个功能化过程中，纳米材料既可以是表面修饰的对象，也可以是用于修饰其他基底表面的材料。这些表面功能化策略，涉及传感器结构和材料的协同作用以及相关的修饰方法，在很大程度上决定了生物传感应用的性能。本文综述介绍了目前对具有多重检测潜力的生物传感器的研究，并特别关注纳米材料在这些生物传感器的设计和功能化中的作用。已详细阐述了用于方法选择的范例，以帮助理解并加速新型纳米技术在生物传感器开发中的应用。
