Schöch G, Garbrecht M, Heller-Schöch G, Baisch H, Leifer W
Blut. 1979 May;38(5):391-6. doi: 10.1007/BF01007900.
The qualitative and quantitative analysis by high performance liquid chromatography of the normal and modiefied nucleobases excreted in urine represents a new and versatile tool, especially in oncology. The excretion of 2 normal (adenine, guanine) and 4 modified nucleobases (methylated guanine derivatives) in urine was measured by cation exchange LC. All chronic myeloproliferative syndromes showed highly elevated total excretion values of all determined nucleobases, the "pattern" being characteristic with N2, N2-dimethylguanine most prominent (up to 29.8 S.D. above the pertinent normal value). A follow-up study of a case of CML with two episodes of extreme leukocytosis showed a correlation of the nucleobases excretion with the number of leukocytes. Thus, a method has been established which permits the assessment of myeloproliferation and probably therapy effects.
通过高效液相色谱法对尿液中排泄的正常和修饰核碱基进行定性和定量分析是一种新的通用工具，尤其在肿瘤学领域。通过阳离子交换液相色谱法测定尿液中2种正常核碱基（腺嘌呤、鸟嘌呤）和4种修饰核碱基（甲基化鸟嘌呤衍生物）的排泄量。所有慢性骨髓增殖性综合征患者所测定的所有核碱基的总排泄值均显著升高，其“模式”具有特征性，其中N2,N2 -二甲基鸟嘌呤最为突出（比相关正常值高出29.8个标准差）。对一例慢性粒细胞白血病（CML）患者进行的两次极度白细胞增多发作的随访研究表明，核碱基排泄与白细胞数量相关。因此，已建立了一种方法，可用于评估骨髓增殖情况以及可能的治疗效果。
