Perveen Nighat, Ahmad Munir
Department of Entomology, University of Agriculture, Faisalabad, Pakistan.
Department of Entomology, PMAS, Arid Agriculture University, Rawalpindi, Pakistan.
Saudi J Biol Sci. 2017 Jul;24(5):1016-1022. doi: 10.1016/j.sjbs.2016.12.011. Epub 2017 Jan 7.
Quantitative studies concerning total and differential haemocyte counts and abnormalities were performed under laboratory conditions for larvae, pupae and adults collected from a wild colony. Haemolymph samples were observed immediately, thirty and sixty minutes after field recommended concentration exposure of five different insecticides. Total haemocyte counts were significantly higher for larvae and pupae but less for adult bees, however, differential haemocyte counts insignificantly different. Exposure of insecticides showed variable response for tested insecticides with immediate increased change with ethofenprox, diafenthiuron and imidacloprid but decreased for all tested insecticides after sixty minutes. For differential haemocyte counts, plasmatocytes and granulocytes increased with exposure of insecticides. Immune response of haemocytes against insecticides showed different degrees of abnormalities like agglutination, denucleation and cell shape distortion. Such studies may help in possible identification of insect defense mechanisms against their exposure to external hazards for instance insecticide exposure.
在实验室条件下，对从野生蜂群采集的幼虫、蛹和成虫进行了全血细胞计数、分类血细胞计数及异常情况的定量研究。在按照田间推荐浓度接触五种不同杀虫剂后，立即、30分钟和60分钟对血淋巴样本进行观察。幼虫和蛹的全血细胞计数显著较高，而成蜂的全血细胞计数较低，不过，分类血细胞计数无显著差异。杀虫剂接触试验显示，受试杀虫剂的反应各不相同，乙虫腈、硫丹和吡虫啉接触后立即出现变化增加，但60分钟后所有受试杀虫剂的变化均减少。对于分类血细胞计数，浆血细胞和粒细胞随杀虫剂接触而增加。血细胞对杀虫剂的免疫反应表现出不同程度的异常，如凝集、去核和细胞形态畸变。此类研究可能有助于识别昆虫抵御外部危害（如接触杀虫剂）的防御机制。
