Liu Hailin, Cao Fuliang, Yin Tongming, Chen Yingnan
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry UniversityNanjing, China.
Front Plant Sci. 2017 Jun 15;8:1041. doi: 10.3389/fpls.2017.01041. eCollection 2017.
L. is a well-known living gymnosperm fossil that has medicinal and ornamental value. In this study, a high density genetic map was constructed with megagametophytes of 94 seeds from a single tree by employing the specific-locus amplified fragment (SLAF) sequencing technique. The average sequencing depth was 11.20×, which yielded 538,031 high-quality SLAFs. Among these SLAFs, 204,361 were heterozygous in the maternal tree and segregated in the progeny. The established map contained 12,263 SLAFs that were assigned to 12 linkage groups (LGs). The number of LGs on this map equaled the number of chromosomes in . The total map length was 1,671.77 cM, with an average distance of 0.89 cM between adjacent marker bins. Map evaluation based on the haplotype map and the heat map validated the high quality of the established map. Because is an economically and biologically important tree, strenuous efforts have been exerted to sequence its genome. This new map, built using sequence-based markers, will serve in the future as a fundamental platform for anchoring sequence assemblies along chromosomes. This map also provides a desirable platform for various genetic studies of , including gene/quantitative trait locus mapping and marker-aided selection.
L.是一种著名的现存裸子植物化石，具有药用和观赏价值。在本研究中，利用特异性位点扩增片段（SLAF）测序技术，以一棵树上94粒种子的雌配子体构建了高密度遗传图谱。平均测序深度为11.20×，共获得538,031个高质量SLAF。在这些SLAF中，有204,361个在母树中为杂合子，并在子代中分离。构建的图谱包含12,263个SLAF，被分配到12个连锁群（LG）。该图谱上LG的数量与L.的染色体数量相等。图谱总长度为1,671.77 cM，相邻标记区间的平均距离为0.89 cM。基于单倍型图谱和热图的图谱评估验证了所构建图谱的高质量。由于L.是一种在经济和生物学上具有重要意义的树种，人们已付出巨大努力对其基因组进行测序。这张使用基于序列的标记构建的新图谱，未来将作为沿着L.染色体锚定序列组装的基础平台。该图谱还为L.的各种遗传研究提供了理想平台，包括基因/数量性状位点定位和标记辅助选择。
