Wang Yunli, Wang Chaojie, Han Hongyu, Luo Yusong, Wang Zhichao, Yan Chundong, Xu Wenlong, Qu Shuping
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs/Northeast Agricultural University, Harbin, China.
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, China.
Front Plant Sci. 2020 Feb 21;10:1782. doi: 10.3389/fpls.2019.01782. eCollection 2019.
Seed traits are agronomically important for breeding, but the genes controlling seed size, seed weight and seed number have not been mapped in (). In this study, 100 F individual derived from two parental lines, "2013-12" and "9-6", were applied to construct a 3,376.87-cM genetic map containing 20 linkage groups (LGs) with an average genetic distance of 0.47 cM using a total of 8,406 specific length amplified fragment (SLAF) markers in . Ten quantitative trait loci (QTLs) of seed width (SW), seed length (SL) and hundred-seed weight (HSW) were identified using the composite interval mapping (CIM) method. The QTLs affecting SW, SL and HSW explained a maximum of 38.6%, 28.9% and 17.2% of the phenotypic variation and were detected in LG6, LG6 and LG17, respectively. To validate these results, an additional 150 F individuals were used for QTL mapping of SW and SL with cleaved amplified polymorphic sequence (CAPS) markers. We found that two major QTLs, SL6-1 and SW6-1, could be detected in both SLAF-seq and CAPS markers in an overlapped region. Based on gene annotation and non-synonymous single-nucleotide polymorphisms (SNPs) in the major SWand SL-associated regions, we found that two genes encoding a VQ motif and an E3 ubiquitin-protein ligase may be candidate genes influencing SL, while an F-box and leucinerich repeat (LRR) domain-containing protein is the potential regulator for SW in C. maxima. This study provides the first high-density linkage map of using SNPs developed by SLAF-seq technology, which is a powerful tool for associated mapping of important agronomic traits, map-based gene cloning and marker-assisted selection (MAS)-based breeding in .
种子性状在育种方面具有重要的农艺学意义，但控制种子大小、种子重量和种子数量的基因尚未在（此处原文缺失具体物种信息）中定位。在本研究中，从两个亲本系“2013 - 12”和“9 - 6”衍生出100个F个体，利用总共8406个特定长度扩增片段（SLAF）标记构建了一张3376.87厘摩的遗传图谱，该图谱包含20个连锁群（LGs），平均遗传距离为0.47厘摩。使用复合区间作图（CIM）方法鉴定了种子宽度（SW）、种子长度（SL）和百粒重（HSW）的10个数量性状位点（QTLs）。影响SW、SL和HSW的QTLs分别在LG6、LG6和LG17中被检测到，它们对表型变异的解释率最高分别为38.6%、28.9%和17.2%。为验证这些结果，另外150个F个体用于用酶切扩增多态性序列（CAPS）标记对SW和SL进行QTL定位。我们发现两个主要的QTLs，SL6 - 1和SW6 - 1，在SLAF - seq和CAPS标记的重叠区域均可被检测到。基于主要的SW和SL相关区域的基因注释和非同义单核苷酸多态性（SNPs），我们发现两个编码VQ基序和一个E3泛素蛋白连接酶的基因可能是影响SL的候选基因，而一个含F - box和富含亮氨酸重复（LRR）结构域的蛋白是南瓜中SW的潜在调控因子。本研究利用SLAF - seq技术开发的SNPs提供了首张（此处原文缺失具体物种信息）的高密度连锁图谱，这是在（此处原文缺失具体物种信息）中进行重要农艺性状关联作图、基于图谱的基因克隆和基于标记辅助选择（MAS）育种的有力工具。
