Molecular Reproductive Medicine, Department of Translational Medicine, Lund University, CRC, Building 91, Plan 10, Jan Waldenströms gata 35, SE 20502, Malmö, Sweden.
Department of Urology, Skåne University Hospital Malmö, Jan Waldenströms gata 7, SE 20502, Malmö, Sweden.
J Assist Reprod Genet. 2017 Oct;34(10):1277-1282. doi: 10.1007/s10815-017-0989-0. Epub 2017 Jun 29.
The purpose of the study was to identify serum microRNAs providing a link between male subfertility and metabolic syndrome (MetS) and validate their diagnostic potential.
Sera were analyzed for fertility and MetS-related parameters in subfertile men (n = 79) and controls (n = 38). Literature review identified miR-155-5p, miR-122-5p, miR-200a-3p, and miR-200c-3p which previously were associated with parameters of fertility as well as metabolic disorders. They were measured in the sera using an absolute quantitation method (qPCR). In order to investigate the value of miRNAs in predicting subfertility, receiver operating characteristic analysis was done.
Subfertile men had higher concentrations of miR-155-5p than controls (p = 0.003) and for miR-200c-3p, the difference was borderline statistically significant (p = 0.05). miR-155-5p and miR-200c-3p were also associated with subfertility in men with no metabolic disturbances (p = 0.008, p = 0.004, respectively). This association was abrogated if any component of MetS was present. The combination of miR-155-5p and miR-200c-3p with follicle-stimulating hormone, being a well-established subfertility parameter, resulted in an overall diagnostic power of AUC = 0.87, which was even higher when men without MetS components were analyzed (AUC = 0.93). Regarding MetS components, statistically significant correlations were found between miR-122-5p and fasting triglycerides, and waist circumference, but no association with subfertility was identified.
Among the four miRNAs analyzed, none of them was associated both with male subfertility and MetS components. The ability of miR-155-5p and miR-200c-3p to identify subfertile men was partly overruled by the presence of metabolic disturbances.
本研究旨在确定血清 microRNAs 与男性不育和代谢综合征（MetS）之间的联系，并验证其诊断潜力。
分析不育男性（n=79）和对照组（n=38）的血清生育和代谢相关参数。文献综述确定了 miR-155-5p、miR-122-5p、miR-200a-3p 和 miR-200c-3p，它们之前与生育参数以及代谢紊乱有关。使用绝对定量法（qPCR）在血清中测量这些 microRNAs。为了研究 microRNAs 在预测不育症中的价值，进行了接收者操作特征分析。
不育男性的 miR-155-5p 浓度高于对照组（p=0.003），miR-200c-3p 的差异具有统计学意义（p=0.05）。miR-155-5p 和 miR-200c-3p 也与无代谢紊乱的男性不育相关（p=0.008，p=0.004）。如果存在 MetS 的任何成分，这种关联就会被消除。将 miR-155-5p 和 miR-200c-3p 与卵泡刺激素相结合，作为一种成熟的不育症参数，得出的整体诊断效能 AUC 为 0.87，如果分析不伴有 MetS 成分的男性，AUC 则更高（0.93）。关于 MetS 成分，发现 miR-122-5p 与空腹甘油三酯和腰围之间存在统计学显著相关性，但与不育症无关。
在分析的四种 microRNAs 中，没有一种与男性不育和 MetS 成分同时相关。miR-155-5p 和 miR-200c-3p 识别不育男性的能力部分被代谢紊乱的存在所推翻。
