Cheng Yang, Nie Jiyun, Li Zhixia, Yan Zhen, Xu Guofeng, Li Haifei, Guan Dikai
Institute of Pomology, Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Fruit (Xingcheng), Ministry of Agriculture, Xingcheng, Liaoning, 125100, China.
Anal Bioanal Chem. 2017 Aug;409(21):5065-5072. doi: 10.1007/s00216-017-0452-1. Epub 2017 Jun 29.
In this study, we have successfully synthesized a forchlorfenuron (CPPU)-imprinted polymer in dimethyl sulfoxide by molecular imprinting technique. In the molecular imprinting, β-cyclodextrin was used as the monomer with 1,6-hexamethylene diisocyanate (HMDI) as the cross-linking agent that formed a complex with forchlorfenuron by inclusion interactions. The adsorption equilibrium was attained in approximately 30 min and the maximum binding capacity was 26.79 mg/g, which indicated that the adsorption kinetics was relatively fast. The results of adsorption and selectivity experiments have shown that the imprinted polymer was able to bind forchlorfenuron specifically and reversibly. The specific recognition of the imprinted polymer for forchlorfenuron resulted from the cooperative effects of inclusion interaction and hydrophobic interaction. This imprinted polymer was also used to accurately determine forchlorfenuron residue in real fruit samples. In the standard spiked strawberry sample, the adsorption recoveries for forchlorfenuron were as high as 90.3, 84.5, and 90.8%, respectively. The binding specificity of the imprinted polymer for forchlorfenuron resulted from the imprinting effect. Therefore, as a reusable material possessing high affinity and selectivity, the imprinted polymer is promising in application to detect pesticide residues in fruit samples. In addition, because β-cyclodextrin is water soluble and can include neutral compounds, this molecularly imprinted polymer can be used to recognize pesticides in aqueous solutions. Graphical abstract Schematic demonstration of molecular imprinting and re-binding of forchlorfenuron.
在本研究中，我们通过分子印迹技术在二甲基亚砜中成功合成了氯吡脲（CPPU）印迹聚合物。在分子印迹过程中，β-环糊精用作单体，1,6-六亚甲基二异氰酸酯（HMDI）作为交联剂，通过包合作用与氯吡脲形成复合物。吸附平衡在大约30分钟内达到，最大结合容量为26.79mg/g，这表明吸附动力学相对较快。吸附和选择性实验结果表明，印迹聚合物能够特异性且可逆地结合氯吡脲。印迹聚合物对氯吡脲的特异性识别源于包合作用和疏水作用的协同效应。这种印迹聚合物还用于准确测定实际水果样品中的氯吡脲残留量。在加标标准草莓样品中，氯吡脲的吸附回收率分别高达90.3%、84.5%和90.8%。印迹聚合物对氯吡脲的结合特异性源于印迹效应。因此，作为一种具有高亲和力和选择性的可重复使用材料，印迹聚合物在检测水果样品中的农药残留方面具有广阔的应用前景。此外，由于β-环糊精可溶于水且能包合中性化合物，这种分子印迹聚合物可用于识别水溶液中的农药。图形摘要：氯吡脲分子印迹与再结合的示意图。
