Fohlmeister I, Hohentanner O
Nat Immun Cell Growth Regul. 1985;4(4):221-8.
The possibility of replacing the space-consuming rat-to-rat colony-forming unit (CFUs) assay by rat-to-mouse assay systems was examined using Wistar rat bone marrow. After considering the published results on the responsiveness of mouse strains to hemopoietic xenografts and on the ways to abrogate "xenogeneic resistance', we tested C57B1/6J and C3H/He mice conditioned by cyclophosphamide (CY) and/or whole-body irradiation in the following combinations: 850 rad C57Bl; 850 rad + CY C57Bl; 800 rad + CY C3H. A linear relationship between the number of cells injected and the macroscopical spleen colony count could be demonstrated with all three combinations. However, we observed a high number of endogenous colonies in the 850 rad C57B1 system. The results were confirmed by karyotype analysis. Colony yield and seeding efficiency with 800 rad + CY C3H were comparable to the rat-to-rat assay, but were considerably lower in the case of 850 rad + CY C57B1. In the latter system, the colonies were primarily erythroid.
利用Wistar大鼠骨髓，研究了用大鼠-小鼠检测系统替代耗空间的大鼠-大鼠集落形成单位（CFU）检测的可能性。在考虑了已发表的关于小鼠品系对造血异种移植的反应性以及消除“异种抗性”方法的结果后，我们测试了经环磷酰胺（CY）和/或全身照射预处理的C57B1/6J和C3H/He小鼠，采用以下组合：850拉德C57Bl；850拉德 + CY C57Bl；800拉德 + CY C3H。所有三种组合都能证明注射细胞数量与宏观脾脏集落计数之间存在线性关系。然而，我们在850拉德C57B1系统中观察到大量内源性集落。核型分析证实了结果。800拉德 + CY C3H的集落产量和接种效率与大鼠-大鼠检测相当，但在850拉德 + CY C57B1的情况下则低得多。在后一种系统中，集落主要是红系的。
