Wu C T, Ru X M, Liu M P, Chu J P
Exp Hematol. 1986 May;14(4):307-11.
By using a spleen colony transfer technique and sex chromosome determinations as a cytogenetic marker, we compared the spleen colony-forming cells of bone marrow origin from three strains of mice, i.e., LACA, C57, and (LACA female X C57 male) F1. The proliferation potential of spleen colony-forming cells was found to be heterogeneous in nature. Some of the cells have the essential characteristics of hemopoietic stem cells (Ps-CFUS), i.e., the capacity for sustained proliferation and differentiation into myeloid and lymphoid lineages of cells, whereas others exhibit the capacity to form gross colonies on irradiated recipient spleen which are capable of differentiating different lineages of blood cells, but have lost the ability to reconstitute hemopoiesis in lethally irradiated recipients (Pg-CFUS). The results of studies on spleen colony-forming cells of F1 mice indicate the existence of a subpopulation of primitive precursors of spleen colony-forming cells (Pre-CFUs) which are not able to form spleen colonies on recipient spleens, but, under appropriate stimuli, may assume the properties of Ps-CFUS. Therefore the sequential process of blood cell differentiation may be described as follows: Pre-CFUS----Ps-CFUS----Pg-CFUS----different lineages of hemopoietic progenitors----different lineages of blood cells.
通过使用脾集落转移技术并将性染色体测定作为细胞遗传学标记，我们比较了来自三种品系小鼠（即LACA、C57以及（LACA雌性×C57雄性）F1）的骨髓来源的脾集落形成细胞。发现脾集落形成细胞的增殖潜力本质上是异质性的。一些细胞具有造血干细胞（Ps-CFUS）的基本特征，即持续增殖并分化为髓系和淋巴系细胞的能力，而其他细胞则表现出在受照射的受体脾脏上形成大集落的能力，这些集落能够分化为不同谱系的血细胞，但已丧失在致死性照射受体中重建造血的能力（Pg-CFUS）。对F1小鼠脾集落形成细胞的研究结果表明存在脾集落形成细胞原始前体（Pre-CFUs）的一个亚群，它们不能在受体脾脏上形成脾集落，但在适当刺激下可能具有Ps-CFUS的特性。因此，血细胞分化的连续过程可描述如下：Pre-CFUS----Ps-CFUS----Pg-CFUS----不同谱系的造血祖细胞----不同谱系的血细胞。
