Institute for Biomedical Sciences, Center for Diagnostics & Therapeutics, Georgia State University, Atlanta, GA 30302, USA.
Food Science & Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA.
Nanomedicine (Lond). 2017 Aug;12(16):1927-1943. doi: 10.2217/nnm-2017-0196. Epub 2017 Jun 30.
To develop novel siRNA delivery system overcoming the limitations of synthetic nanoparticles, such as potential side effects, nonspecificity and economic production for ulcerative colitis therapy.
MATERIALS & METHODS: Nanoparticles composed of edible ginger-derived lipid, termed ginger-derived lipid vehicles (GDLVs) were generated from ginger lipids through hydration of a lipid film, a commonly used method for a liposome fabrication. The morphology, biocompatibility and transfection efficiency of GDLVs loaded with siRNA-CD98 (siRNA-CD98/GDLVs) were characterized by standard methods.
Orally administered siRNA-CD98/GDLVs were effectively targeted specifically to colon tissues, resulting in reduced expression of CD98.
These GDLVs have great promise as efficient siRNA-delivery vehicles while potentially obviating issues related to the traditional synthetic nanoparticles. As such, they help shift the current paradigm of siRNA delivery away from artificially synthesized nanoparticles toward the use of naturally derived nanovehicles from edible plants.
开发克服合成纳米颗粒局限性的新型 siRNA 递药系统，例如用于溃疡性结肠炎治疗的潜在副作用、非特异性和经济生产。
通过水合脂质膜从姜脂中生成由可食用姜衍生脂质组成的纳米颗粒，即姜衍生脂质载体（GDLV），这是一种常用于脂质体制备的方法。通过标准方法对负载 siRNA-CD98 的 GDLV（siRNA-CD98/GDLV）进行形态、生物相容性和转染效率的表征。
经口服给予的 siRNA-CD98/GDLV 可特异性靶向结肠组织，从而降低 CD98 的表达。
这些 GDLV 有望成为高效的 siRNA 递药载体，同时可能避免与传统合成纳米颗粒相关的问题。因此，它们有助于将 siRNA 递药的当前范式从人工合成纳米颗粒转向使用来自食用植物的天然衍生纳米载体。
