Liu Jingjing, Deng Wenjie, Yu Muqian, Wen Ruizhi, Yao Shouzhuo, Chen Bo
Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China; College of Chemistry and Chemical Engineering, University of South China, Hengyang, 421001, China.
Key Laboratory of Phytochemical R&D of Hunan Province and Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research, Ministry of Education, Hunan Normal University, Changsha 410081, China.
J Pharm Biomed Anal. 2017 Oct 25;145:151-157. doi: 10.1016/j.jpba.2017.06.030. Epub 2017 Jun 20.
A paper spray mass spectrometry (PS-MS) method for rapid and reliable analysis of benzalkonium chloride (BAC) in compound eye drops and body surface disinfectant was developed. The sample was dropped onto triangular filter paper, and high voltage (3.5kV) was applied to form an electrospray. This method can provide the composition of benzalkonium chloride in samples without pretreatment, solvent or chromatographic separation, and the analysis time is only 10s. The primary homologues C-BAC, C-BAC and C-BAC of benzalkonium chloride were quantitatively analyzed using PS-MS. Samples were subjected to simple dilution and quantified using the internal standard method. Ion trap mass spectrometry was scanned using SIM mode. The linear ranges of C-BAC, C-BAC and C-BAC were 1-100μgmL; the linear regression coefficients were 0.998-0.999; the detection limits (LODs) were 0.1μgmL; the limit of quantifications (LOQs) was <1μgmL, and the method validation indicated that the method precision and accuracy were good. Compared with HPLC-UV methods, there was no significant difference in the quantitative determination of the actual samples, but the analysis time for PS-MS is shorter (2min). In addition, reagent consumption in PS-MS is small, and no chromatographic separation is needed, suggesting that PS-MS is especially suitable for high-throughput analysis.
建立了一种用于快速可靠分析复方滴眼液和体表消毒剂中苯扎氯铵（BAC）的纸喷雾质谱（PS-MS）方法。将样品滴加到三角形滤纸上，并施加高电压（3.5kV）以形成电喷雾。该方法无需预处理、溶剂或色谱分离即可提供样品中苯扎氯铵的组成，分析时间仅为10秒。使用PS-MS对苯扎氯铵的主要同系物C-BAC、C-BAC和C-BAC进行了定量分析。样品进行简单稀释并采用内标法进行定量。采用SIM模式扫描离子阱质谱。C-BAC、C-BAC和C-BAC的线性范围为1-100μg/mL；线性回归系数为0.998-0.999；检测限（LOD）为0.1μg/mL；定量限（LOQ）<1μg/mL，方法验证表明该方法的精密度和准确度良好。与HPLC-UV方法相比，实际样品的定量测定无显著差异，但PS-MS的分析时间更短（2分钟）。此外，PS-MS的试剂消耗量小，无需色谱分离，表明PS-MS特别适用于高通量分析。
