Laboratory of Environmental Biotechnology, Faculty of Environment and Resource Studies, Mahidol University, Salaya, Nakhonpathom, 73170, Thailand.
Environ Sci Pollut Res Int. 2017 Aug;24(23):19249-19258. doi: 10.1007/s11356-017-9591-3. Epub 2017 Jun 30.
This study examined the performance of the chitosan-immobilized cadmium-resistant bacteria Arthrobacter sp. and Micrococcus sp. on cadmium phytoremediation by Chlorophytum laxum in cadmium-polluted soil. These immobilized cadmium-resistant bacteria can survive in cadmium-contaminated soil and significantly increased soil cadmium solubility, but the ability of chitosan-immobilized cells to increase cadmium solubility was lower than that of free cells. A pot experiment demonstrated that chitosan-immobilized Micrococcus sp. promoted the growth of C. laxum planted in cadmium-contaminated soil. A significant increase in the cadmium concentration in the roots and aboveground parts of C. laxum was found in plants inoculated with free and chitosan-immobilized cells of these bacteria. The performance of Arthrobacter sp. free cells to augment cadmium accumulation in C. laxum was a little bit better than that of chitosan-immobilized Arthrobacter sp., except at 9 weeks after planting. The phytoextraction coefficient, bioaccumulation factor, and translocation factor of C. laxum inoculated with free and chitosan-immobilized cells of cadmium-resistant bacteria were higher than those of the uninoculated control and increased with time. Our findings suggest that chitosan-immobilized cells can be exploited to enhance the efficiency of cadmium phytoremediation by C. laxum.
本研究考察了壳聚糖固定化耐镉菌节杆菌和微球菌在镉污染土壤中通过吊兰进行植物修复的性能。这些固定化耐镉菌可以在镉污染土壤中存活，并显著提高土壤中镉的溶解度，但壳聚糖固定化细胞提高镉溶解度的能力低于游离细胞。盆栽实验表明，壳聚糖固定化微球菌促进了种植在镉污染土壤中的吊兰的生长。在接种这些细菌的游离细胞和壳聚糖固定化细胞的植物中，发现吊兰的根和地上部分的镉浓度显著增加。游离节杆菌细胞增强吊兰中镉积累的性能略优于壳聚糖固定化节杆菌细胞，但在种植后 9 周时除外。接种游离和壳聚糖固定化耐镉菌细胞的吊兰的植物萃取系数、生物积累系数和转运系数均高于未接种对照，且随时间增加而增加。我们的研究结果表明，壳聚糖固定化细胞可以被利用来提高吊兰进行镉植物修复的效率。
