Laboratory of Environmental Biotechnology, Faculty of Environment and Resource Studies, Mahidol University, Salaya, Nakhon Pathom, 73170, Thailand.
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Environ Sci Pollut Res Int. 2021 Aug;28(29):39398-39408. doi: 10.1007/s11356-021-13527-z. Epub 2021 Mar 23.
This work aims to explore the role of cadmium-resistant actinomycetes on promoting plant growth and cadmium uptake in Chlorophytum comosum (Thunb.) Jacques, a spider plant. Actinomycetes isolated from the plant roots in peat swamp forests were screened for their cadmium resistance and the production of indole-3-acetic acid (IAA) and siderophores. The results found that K5PN1 and 11-10SHTh produced high levels of IAA and siderophores, respectively. K5PN1 and 11-10SHTh were identified to be Streptomyces rapamycinicus and Streptomyces cyaneus, respectively. Both strains were able to remove cadmium from aqueous solution and survive under cadmium stress in contaminated soil. The results of pot experiments found that the selected Streptomyces inoculation increased the root and shoot biomass and cadmium accumulation in the root and shoot of C. comosum planted in a cadmium-contaminated soil. The highest cadmium accumulation and translocation ability of cadmium from the root to shoot was found in C. comosum with S. rapamycinicus inoculation. In addition, plant with S. cyaneus inoculation had the highest phytoextraction coefficient and bioaccumulation factor. Our findings concluded that S. rapamycinicus and S. cyaneus stimulated the growth and cadmium uptake in C. comosum, suggesting a combined approach using the selected Streptomyces and C. comosum for phytoremediation of cadmium-polluted soil.
本研究旨在探索镉抗性放线菌在促进吊兰（Chlorophytum comosum (Thunb.) Jacques）生长和镉吸收中的作用。从泥炭沼泽森林植物根系中分离出的放线菌进行了镉抗性和吲哚-3-乙酸（IAA）和铁载体生产的筛选。结果发现，K5PN1 和 11-10SHTh 分别产生高水平的 IAA 和铁载体。K5PN1 和 11-10SHTh 分别被鉴定为瑞氏链霉菌（Streptomyces rapamycinicus）和蓝色链霉菌（Streptomyces cyaneus）。这两种菌株都能够从水溶液中去除镉，并在污染土壤中的镉胁迫下存活。盆栽实验结果发现，选择的链霉菌接种增加了种植在镉污染土壤中的吊兰的根和茎生物量以及根和茎中的镉积累。在接种 S. rapamycinicus 的吊兰中，镉从根部向地上部的积累和转运能力最高。此外，接种 S. cyaneus 的植物具有最高的植物萃取系数和生物累积因子。我们的研究结果表明，S. rapamycinicus 和 S. cyaneus 刺激了 C. comosum 的生长和镉吸收，这表明可以使用选定的链霉菌和 C. comosum 联合进行镉污染土壤的植物修复。
