Ketaubon Patipat, Prapagdee Benjaphorn
Faculty of Environment and Resource Studies, Mahidol University, Salaya, Nakhon Pathom, 73170, Thailand.
Environ Sci Pollut Res Int. 2023 Nov;30(52):113015-113025. doi: 10.1007/s11356-023-30382-2. Epub 2023 Oct 17.
This study aims to formulate bacterial tablets of cadmium (Cd)-resistant Micrococcus sp. MU1, an indole-3-acetic acid-producer, for soil inoculation to improve Cd phytoremediation by Chlorophytum comosum (Thunb.) Jacques. The viability of Micrococcus sp. MU1 in tablets after storage at room temperature and 4 °C was determined. The ability of Micrococcus sp. tablets and cell suspensions on stimulating growth and Cd accumulation in C. comosum was compared. The results found that the viability of Micrococcus sp. tablets stored at room temperature and 4 °C for 2 months were 29.2 and 97.9%, respectively. After 2 months of growth in pots, the dry biomass weights of C. comosum amended with Micrococcus sp. tablet and cell suspension were greater than that of uninoculated control by 1.4- and 1.3-fold, respectively. Cd concentrations in the roots and shoots of C. comosum inoculated with bacterial tablet and bacterial suspension were not significantly different (p < 0.05) and were greater than that of the uninoculated plants. In addition, plants inoculated with Micrococcus sp. tablet and cell suspension exhibited superior phytoextraction performance, bioaccumulation factor, and translocation factor, indicating equal performance of both bacterial forms on boosting Cd phytoremediation efficiency in C. comosum. These findings suggest that soil inoculation with Micrococcus sp. tablet as a ready-to-use inoculum is a novel approach to promote phytoremediation of C. comosum in Cd-contaminated agricultural soil.
本研究旨在制备由产吲哚 - 3 - 乙酸的抗镉微球菌属MU1制成的菌剂用于土壤接种，以提高吊兰对镉的植物修复能力。测定了微球菌属MU1菌剂在室温及4℃储存后的活力。比较了微球菌属菌剂和细胞悬液对吊兰生长及镉积累的促进能力。结果发现，微球菌属菌剂在室温及4℃储存2个月后的活力分别为29.2%和97.9%。盆栽生长2个月后，用微球菌属菌剂和细胞悬液处理的吊兰干生物量分别比未接种对照高1.4倍和1.3倍。接种菌剂和细菌悬液的吊兰根和地上部的镉浓度无显著差异（p < 0.05），且高于未接种植物。此外，接种微球菌属菌剂和细胞悬液的植物表现出更好的植物提取性能、生物积累系数和转运系数，表明两种细菌形式在提高吊兰对镉的植物修复效率方面性能相当。这些发现表明，用微球菌属菌剂作为即用型接种物进行土壤接种是一种促进镉污染农田土壤中吊兰植物修复的新方法。
