Williamson D H, Ilic V
Biochem J. 1985 Nov 1;231(3):773-5. doi: 10.1042/bj2310773.
The activities of two mitochondrial enzymes concerned in the utilization of acetoacetate, namely 3-oxoacid CoA-transferase and acetoacetyl-CoA thiolase, were high throughout the suckling and weanling period in brown adipose tissue of the rat. In contrast, 3-hydroxybutyrate dehydrogenase activity was comparatively low during this period. The activity of cytosolic acetoacetyl-CoA synthetase (involved in lipogenesis) declined after birth and remained low until the pups were weaned. Experiments with brown-adipose-tissue slices from weanling rats indicated that 70% of the [3-14C]acetoacetate utilized was oxidized to 14CO2, and this value was not altered appreciably by the addition of glucose and insulin.
与乙酰乙酸利用相关的两种线粒体酶，即3-氧代酸辅酶A转移酶和乙酰乙酰辅酶A硫解酶，在大鼠棕色脂肪组织的整个哺乳期和断奶期活性都很高。相比之下，在此期间3-羟基丁酸脱氢酶活性相对较低。胞质乙酰乙酰辅酶A合成酶（参与脂肪生成）的活性在出生后下降，并一直保持在低水平，直到幼崽断奶。对断奶大鼠棕色脂肪组织切片进行的实验表明，所利用的[3-14C]乙酰乙酸中有70%被氧化为14CO2，添加葡萄糖和胰岛素后，这一数值没有明显变化。
