海人酸诱导的乌龟视网膜中 OFF 双极细胞释放血清素。

Kainic acid-induced release of serotonin from OFF-bipolar cells in the turtle retina.

作者信息

Weiler R, Schütte M

出版信息

Brain Res. 1985 Dec 23;360(1-2):379-83. doi: 10.1016/0006-8993(85)91259-4.

Abstract

The immunofluorescence of antibody-labelled serotonin was used as an indicator for the amount of intracellular serotonin. Incubation in Ringer solution containing serotonin and pargyline increased the immunofluorescence which was subsequently decreased in a calcium-dependent manner by incubation in high-potassium Ringer. If preloaded retinas were exposed to 10 microM kainic acid, the immunofluorescence decreased in a time-dependent way. This kainic acid-induced release was blocked by piperidine dicarboxylic acid. These results support the idea that serotonin is a transmitter in OFF-bipolar cells.

摘要

抗体标记血清素的免疫荧光被用作细胞内血清素含量的指标。在含有血清素和帕吉林的林格溶液中孵育会增加免疫荧光，随后通过在高钾林格溶液中孵育以钙依赖的方式降低免疫荧光。如果将预先加载的视网膜暴露于10微摩尔红藻氨酸，免疫荧光会随时间下降。这种红藻氨酸诱导的释放被哌啶二羧酸阻断。这些结果支持血清素是双极无长突细胞中的一种神经递质的观点。