McCalley David V
Centre for Research in Biosciences, University of the West of England, Frenchay, Bristol BS16 1QY, UK.
J Chromatogr A. 2017 Nov 10;1523:49-71. doi: 10.1016/j.chroma.2017.06.026. Epub 2017 Jun 13.
Hydrophilic interaction liquid chromatography (HILIC) has emerged as a valuable complimentary technique to reversed-phase (RP), being especially suited for the analysis of polar and ionised solutes, which are difficult to retain in RP. For solutes amenable to both separation mechanisms, HILIC provides a different selectivity to RP, and also offers possibilities as an orthogonal mechanism for 2-dimensional LC when combined with RP. HILIC has further advantages of lower column back pressures, and increased sensitivity with mobile phase evaporative detectors such as electrospray mass spectrometry. This review covers progress in our understanding of the HILIC technique, principally over the last ten years, including the classification of columns, the factors that control retention and selectivity, and attempts to model the separation process and its kinetics.
亲水作用液相色谱法（HILIC）已成为反相色谱法（RP）一种有价值的补充技术，特别适用于分析在反相色谱中难以保留的极性和离子化溶质。对于适用于两种分离机制的溶质，HILIC对反相色谱提供了不同的选择性，并且与反相色谱结合时，作为二维液相色谱的正交机制也提供了可能性。HILIC还有柱背压较低以及使用电喷雾质谱等流动相蒸发检测器时灵敏度增加等优点。本综述涵盖了我们对HILIC技术理解方面的进展，主要是过去十年间的进展，包括色谱柱的分类、控制保留和选择性的因素，以及对分离过程及其动力学进行建模的尝试。
