非癌组织中血小板反应蛋白-1表达与胃癌发生的相关性

Correlation Between Thrombospondin-1 Expression in Non-cancer Tissue and Gastric Carcinogenesis.

作者信息

Kashihara Hideya, Shimada Mitsuo, Yoshikawa Kozo, Higashijima Jun, Tokunaga Takuya, Nishi Masaaki, Takasu Chie, Ishikawa Daichi

机构信息

Department of Surgery, Institute of Health Biosciences, University of Tokushima, Tokushima, Japan

Department of Surgery, Institute of Health Biosciences, University of Tokushima, Tokushima, Japan.

出版信息

Anticancer Res. 2017 Jul;37(7):3547-3552. doi: 10.21873/anticanres.11724.

DOI:10.21873/anticanres.11724

PMID:28668845

Abstract

BACKGROUND/AIM: Thrombospondin-1 (TSP1) is correlated with carcinogenesis occurring in cases of intestinal inflammation. The aim of this study was to clarify the role of TSP1 in gastric carcinogenesis.

MATERIALS AND METHODS

A total of 39 patients with gastric cancer who had undergone gastrectomy were enrolled. The expression of TSP1 mRNA in non-cancer tissues was determined. Furthermore, the expression of CD36, STAT3 and TGFβR2 mRNA in non-cancer tissues in two expression groups, the TSP1 high- and low-expression groups, were examined.

RESULTS

The expression of TSP1 was high in the mucosal-atrophy group and tended to be high in the Helicobacter pylori (H. pylori) (+) and multiple cancer groups. The levels of CD36, STAT3 and TGFβR2 mRNA were significantly higher in the TSP1-high group. TSP1 signaling pathway was induced in multiple cancer or atrophy (+) or H. pylori (+) compared to cases with single cancer, atrophy (-) and H. pylori (-). Expression of proteins involved in the TSP1 signaling pathway in non-cancer tissues with multiple gastric cancers were higher than that with single gastric cancer.

CONCLUSION

Expression of TSP1 in non-cancer tissue correlated with gastric carcinogenesis.

摘要

背景/目的：血小板反应蛋白-1（TSP1）与肠道炎症相关的致癌作用有关。本研究的目的是阐明TSP1在胃癌发生中的作用。

材料与方法

共纳入39例行胃切除术的胃癌患者。测定非癌组织中TSP1 mRNA的表达。此外，检测TSP1高表达组和低表达组两个表达组中非癌组织中CD36、STAT3和TGFβR2 mRNA的表达。

结果

TSP1在黏膜萎缩组中表达较高，在幽门螺杆菌（H. pylori）阳性组和多癌组中也有表达升高的趋势。TSP1高表达组中CD36、STAT3和TGFβR2 mRNA水平显著更高。与单发癌、萎缩阴性和幽门螺杆菌阴性的病例相比，多癌、萎缩阳性或幽门螺杆菌阳性的病例中TSP1信号通路被诱导。多原发性胃癌非癌组织中TSP1信号通路相关蛋白的表达高于单发性胃癌。