LAMB1 与 T 分期相关，提示胃癌预后不良。
LAMB1 Is Related to the T Stage and Indicates Poor Prognosis in Gastric Cancer.
机构信息
Department of Gastroenterology and Hepatology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
The Fourth Department of Infectious Disease, Chongqing Public Health Center, Chongqing, China.
出版信息
Technol Cancer Res Treat. 2021 Jan-Dec;20:15330338211004944. doi: 10.1177/15330338211004944.
BACKGROUND AND OBJECTIVE
Gastric cancer (GC) is a common tumor malignancy with high incidence and poor prognosis. Laminin is an indispensable component of basement membrane and extracellular matrix, which is responsible for bridging the internal and external environment of cells and transmitting signals. This study mainly explored the association of the LAMB1 expression with clinicopathological characteristics and prognosis in gastric cancer.
METHODS
The expression data and clinical information of gastric cancer patients were downloaded from The Cancer Genome Atlas (TCGA) and Asian Cancer Research Group (ACRG). And we analyzed the relationship between LAMB1 expression and clinical characteristics through R. CIBERSORTx was used to calculate the absolute score of immune cells in gastric tumor tissues. Then COX proportional hazard models and Kaplan-Meier curves were performed to evaluate the role of LAMB1 and its influence on prognosis in gastric cancer patients. Finally, GO and KEGG analysis were applied for LAMB1-related genes in gastric cancer, and PPI network was constructed in Cytoscape software.
RESULTS
In the TCGA cohort, patients with gastric cancer frequently generated LAMB1 gene copy number variation, but had little effect on mRNA expression. Both in the TCGA and ACRG cohorts, the mRNA expression of LAMB1 in gastric cancer tissues was higher than it in normal tissues. All patients were divided into high expression group and low expression group according to the median expression level of LAMB1. The elevated expression group obviously had more advanced cases and higher infiltration levels of M2 macrophages. COX proportional hazard models and Kaplan-Meier curves revealed that patients with enhanced expression of LAMB1 have a worse prognosis. GO/KEGG analysis showed that LAMB1-related genes were enriched in PI3K-Akt signaling pathway, focal adhesion, ECM-receptor interaction, etc.
CONCLUSIONS
The high expression of LAMB1 in gastric cancer is related to the poor prognosis of patients, and it may be related to microenvironmental changes in tumors.
背景与目的
胃癌（GC）是一种常见的肿瘤恶性肿瘤，发病率高，预后差。层粘连蛋白是基底膜和细胞外基质的不可或缺组成部分，负责桥接细胞的内外环境并传递信号。本研究主要探讨了 LAMB1 表达与胃癌临床病理特征和预后的关系。
方法
从癌症基因组图谱（TCGA）和亚洲癌症研究组（ACRG）下载了胃癌患者的表达数据和临床信息。我们通过 R 分析了 LAMB1 表达与临床特征之间的关系。使用 CIBERSORTx 计算胃肿瘤组织中免疫细胞的绝对评分。然后进行 COX 比例风险模型和 Kaplan-Meier 曲线分析，以评估 LAMB1 在胃癌患者中的作用及其对预后的影响。最后，在胃癌中进行了 LAMB1 相关基因的 GO 和 KEGG 分析，并在 Cytoscape 软件中构建了 PPI 网络。
结果
在 TCGA 队列中，胃癌患者经常发生 LAMB1 基因拷贝数变异，但对 mRNA 表达影响不大。在 TCGA 和 ACRG 队列中，胃癌组织中 LAMB1 的 mRNA 表达均高于正常组织。根据 LAMB1 的中位表达水平，所有患者均分为高表达组和低表达组。高表达组的病例明显更晚期，M2 巨噬细胞浸润水平更高。COX 比例风险模型和 Kaplan-Meier 曲线表明，LAMB1 表达增强的患者预后较差。GO/KEGG 分析表明，LAMB1 相关基因富集于 PI3K-Akt 信号通路、焦点黏附、ECM-受体相互作用等通路。
结论
胃癌中 LAMB1 的高表达与患者的不良预后有关，可能与肿瘤微环境变化有关。
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